卡马西平对癫痫模型大鼠海马损伤作用及对Fascin-1、突触素表达的影响  

Effects of Carbamazepine on Hippocampal Injury and Expression of Fascin-1 and Synaptophysin in Epileptic Rats

作　　者：朱百科 王新新 ZHU Baike;WANG Xinxin(Shandong Provinical Third Hospital,Jinan 250031,Shandong,China)

机构地区：[1]山东省立第三医院,济南250031 [2]济南市第五人民医院

出　　处：《中西医结合心脑血管病杂志》2021年第7期1108-1112,共5页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

摘　　要：目的研究卡马西平对癫痫模型大鼠海马损伤的作用及对肌成束蛋白(Fascin-1)、突触素(SYP)表达的影响。方法选择54只无特定病原体动物(SPF)级SD健康大鼠,选取40只建立癫痫模型,分为模型组、低剂量组、中剂量组、高剂量组,每组10只,另设10只正常大鼠为正常组。低剂量组、中剂量组、高剂量组分别给予卡马西平(质量浓度2 g/L)15 mg/kg、30 mg/kg、50 mg/kg进行治疗,采用脑电图机观察大鼠潜伏期、持续时间、波幅指标,统计发作次数、发作总时间,苏木精-伊红染色法(HE)染色处理,观察海马区细胞凋亡指数,采用蛋白质印迹法(Western Blot)检测Fascin-1、突触素、Bcl-2、Bax表达水平。结果模型组潜伏期及Fascin-1、Bcl-2表达水平低于正常组、低剂量组、中剂量组、高剂量组,持续时间、波幅、海马区细胞凋亡指数及突触素、Bax表达水平高于正常组、低剂量组、中剂量组、高剂量组,差异均有统计学意义(P<0.05)。卡马西平各剂量组潜伏期高于模型组,持续时间、波幅、海马区细胞凋亡指数低于模型组,差异具有统计学意义(P<0.05)。中剂量组潜伏期、Fascin-1、Bcl-2表达水平高于低剂量组和高剂量组,持续时间、波幅、发作次数、发作总时间、海马区细胞凋亡指数及突触素、Bax表达水平低于低剂量组和高剂量组,差异有统计学意义(P<0.05)。结论卡马西平能降低癫痫模型大鼠海马损伤程度,起到一定的抗癫痫作用,可能与调控Fascin-1、突触素、Bcl-2、Bax表达水平有关。Objective To investigate effects of carbamazepine on hippocampal injury and expression of Fascin-1 and synaptophysin(SYP)in epileptic rats.Methods Fifty-four healthy SD rats of specific pathogen free(SPF)were selected and 40 epileptic models were established.A fourty rats were divided into model group,low dose group,middle dose group,high dose group and normal group,with 10 rats in each group.Low dose group,middle dose group,and high dose group were respectively treated with carbamazepine(mass concentration 2 g/L)15 mg/kg,30 mg/kg and 50 mg/kg of body weight.The latency,duration and amplitude indexes ofrats were observed by electroencephalograph,ologram the number and total duration of seizures were counted.The apoptosis index of hippocampal cells was observed by hematoxylin-eosin(HE)staining.The expression of Fascin-1,SYP,Bcl-2 and Bax were detected by Western Blotting.Results The expressions of latency,Fastin-1,Bcl-2 in the model group were lower than those in the normal group,the low dose group,the middle dose group,and the high dose group.The duration,amplitude,apoptosis index,SYP,Bax in the model group were more than those in the normal group,the low dose group,the middle dose group and the high dose group(P<0.05).The incubation period in carbamazepine group was higher than that of model group,and the duration,amplitude and apoptosis index of hippocampus were lower than those of model group(P<0.05).The expression of latency,Fastin-1 and Bcl-2 in the middle dose group were more than those in low dose group and high dose group,while the expression of duration,amplitude,attack times,total attack time,apoptosis index,SYP and Bax in hippocampus were less than those in the low dose group and the high dose group(P<0.05).Conclusion Carbamazepine could reduce the degree of hippocampal damage in epileptic rats and play a certain antiepileptic effect,which might be related to the regulation of Fascin-1,SYP,Bcl-2,Bax.

关 键 词：癫痫 卡马西平 海马区 肌成束蛋白 突触素 实验研究 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]