超声靶向微泡破坏技术协同二氢卟吩e6介导声动力治疗小鼠肝癌移植瘤  

The Inhibitory Effect of Ultrasound-targeted Microbubble Destruction Combined with Chlorin e6-mediated Sonodynamic Therapy on Mice Hepatocellular Carcinoma

作　　者：谭妍迪 刘朝奇[1] 赵云[1] 周军[2] 谢茜 李金林 Tan Yandi;Liu Chaoqi;Zhao Yun;Zhou Jun;Xie Qian;Li Jinlin(Medical College of China Three Gorges University,Yichang,Hubei 443002,China;Yichang Central People's Hospital,Yichang,Hubei 443002,China)

机构地区：[1]三峡大学医学院,湖北省宜昌市443002 [2]宜昌市中心人民医院超声科,湖北省宜昌市443002

出　　处：《中国超声医学杂志》2021年第4期467-469,共3页Chinese Journal of Ultrasound in Medicine

摘　　要：目的探讨载二氢卟吩e6纳米脂质微泡介导的超声靶向微泡破坏技术及声动力治疗对肝癌移植瘤的协同抑制作用。方法制备Ce6-MB并检测其形态、粒径及包封率。将H22肝癌皮下移植瘤小鼠随机分为对照组、Empty-MB组、Ce6组、Ce6-MB组。治疗5次后计算抑瘤率,冰冻切片观察Ce6肿瘤聚集情况,HE、TUNEL染色观察细胞及组织凋亡情况,RT-qPCR及免疫组化法检测Bcl-2、Bax的基因及蛋白表达。结果 Ce6-MB为分散均匀纳米级球形微泡,包封率为40.85%±3.09%,Ce6-MB组比Ce6组肿瘤聚集更多Ce6。与对照组相比,Empty-MB、Ce6、Ce6-MB组均可抑制肿瘤生长,Bax mRNA和蛋白高表达,Bcl-2低表达,以上结果以Ce6-MB组为显著(P<0.01)。结论成功制备载Ce6纳米脂质微泡,超声靶向微泡破坏技术协同Ce6介导的声动力疗法可有效治疗小鼠肝癌移植瘤。Objective This study evaluated the synergistic therapeutic effect of Ce6-MB mediated ultrasound-targeted microbubble destruction and sonodynamic therapy on transplanted hepatocellular carcinoma. Methods Ce6-MB was prepared and its morphological characterization, average diameter, and drug-loading capacity were measured. H22 hepatocellular carcinoma xenograft mice were randomly divided into the Control group, Empty-MB group, Ce6 group, and Ce6-MB group. After five-times treatments, the tumor inhibition rate, the fluorescence intensity of chlorin e6 in the tumor, tumor pathological examination, apoptotic cells, the mRNA and protein expressions of Bcl-2, Bax were observed. Results The prepared Ce6-MB was spherical and dispersed uniformly. The drug-loading capacity was 40.85%±3.09%. Compared with the Control group, the treatment groups showed the effective antitumor activity. The gene and protein expression levels of Bax were higher, while the results of Bcl-2 were reversed, the best effect could be observed in the Ce6-MB group(P<0.01). Conclusions We successfully fabricated Ce6 microbubble. Ce6-MB mediated ultrasound-targeted microbubble destruction combine SDT could achieve synergistic therapeutic effects on mice H22 hepatocellular carcinoma.

关 键 词：微泡 声动力治疗 二氢卟吩e6 超声靶向微泡破坏技术:肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]