产羧甲基纤维素酶的 Bacillus sp.B59培养优化研究  

Culture Optimization of Bacillus sp.B59for the Production of Carboxymethyl-cellulase

作　　者：仉倩 马瑞梅 解孝满 董全行 葛宜和[1] 冯志彬[1] 程仕伟[1] ZHANG Qian;MA Ruimei;XIE Xiaoman;DONG Quanxing;Ge Yihe;Feng Zhibin;CHENG Shiwei(School of Life Sciences,Ludong University,Yantai 264025,China;Shandong Forest Germplasm Resources Center,Jinan 265709,China)

机构地区：[1]鲁东大学生命科学学院,山东烟台264039 [2]山东省林木种质资源中心,济南265709

出　　处：《鲁东大学学报（自然科学版）》2021年第2期146-150,156,共6页Journal of Ludong University:Natural Science Edition

基　　金：山东省自然科学基金(ZR2019MC037);山东省重点研发计划(2018GNC113013);山东省农业重大应用技术创新项目(鲁财指2014-38)。

摘　　要：芽孢杆菌来源纤维素酶具有耐热耐碱等优良特性,在饲料添加剂等行业具有重要应用。本文以分离自近海土壤的芽孢杆菌(Bacillus sp.)B59为研究对象,通过优化碳源、氮源、金属盐、初始pH值、培养温度和接种量对菌株产羧甲基纤维素酶的影响,正交试验确定最佳产酶工艺为:2 g·L^(-1)果糖,20 g·L^(-1)蛋白胨,0.5 g·L^(-1) FeCl _(3),3 g·L-1 ZnSO_(4),0.2 g·L^(-1)羧甲基纤维素,pH值8.5,接种量1%(v/v),培养温度28℃。在优化条件下发酵培养40 h,获得羧甲基纤维素酶活力为786.92 U·mL^(-1),为后续的规模制备及应用奠定基础。Cellulase from Bacillus has excellent properties such as heat and alkaline resistance,that resulted in important applications in feed additives and other industries.In this study,using Bacillus sp.B59 isolated from the offshore soil,the influencing factors of carboxymethyl-cellulase(CMCase)producing was studied by optimizing carbon source,nitrogen source,metal salt,initial pH,culture temperature and inoculation amount,and then the orthogonal test was used to determine the optimum CMCase production process,which was 25 g·L^(-1 )fructose,20 g·L^(-1) peptone,0.5 g·L^(-1 )FeCl_(3),3 g·L^(-1) ZnSO4,0.2 g·L^(-1) carboxymethyl-cellulose,pH 8.5,1%(v/v)inoculation amount and culture temperature of 28℃.The maximum CMCase activity was obtained as 786.92 U·mL^(-1) under optimal culture conditions for 40 h.This study lays a foundation for the subsequent scale preparation and application.

关 键 词：芽孢杆菌 羧甲基纤维素酶 培养优化 发酵生产 

分 类 号：Q556[生物学—生物化学]