大亚湾海域4种常见危化品对发光细菌的生物毒性研究  

Study on biological acute toxicity of four common hazardous chemicals to luminescent bacteria in Daya bay

作　　者：袁一鸣[1,2] 王腾 何彦龙 杨涛[1,2] 黄元洲 李志恩[1,2] 张昊飞 YUAN Yi-ming;WANG Teng;HE Yan-long;YANG Tao;HUANG Yuan-zhou;LI Zhi-en;ZHANG Hao-fei(East China Sea Environmental Monitoring Center,SOA,Shanghai 201206,China;Key Laboratory of marine ecological monitoring and restoration technology,MNR,Shanghai 201206,China)

机构地区：[1]国家海洋局东海环境监测中心,上海201206 [2]自然资源部海洋生态监测与修复技术重点实验室,上海201206

出　　处：《海洋环境科学》2021年第2期235-241,共7页Marine Environmental Science

基　　金：国家重点研发计划项目(2017YFC1404500);惠州市2017年市级统筹柴油补贴资金项目(G2017-01);自然资源部海洋生态监测与修复重难点实验室课题基金项目(MATHAB201826);自然资源部东海局青年科技基金项目(2019001)。

摘　　要：采用发光细菌法对大亚湾海域海水进行生物急性毒性实验,掌握大亚湾海域海水水质情况,并对大亚湾海域海水进行4种危化品生物急性毒性实验。结果显示,7月和11月大亚湾海域各站位海水对发光细菌的抑制率均小于30%,为低度毒性风险。4种危化品生物急性毒性实验结果显示,甲醛对发光细菌的半抑制浓度IC50为8.609 mg/L,甲醇对发光细菌的IC_(50)为0.048 mg/L,甲苯对发光细菌的IC_(50)为0.002 mg/L,苯酚对发光细菌的IC_(50)为43.484 mg/L,4种危化品对发光细菌的IC_(50)为甲苯<甲醇<甲醛<苯酚。In order to master the sea water quality of Daya bay,the biological toxicity experiment was carried out in Daya bay by using luminescent bacteria method. The luminescent bacteria were also employed to detect the acute toxicity of four kinds of hazardous chemicals by simulating the leakage of hazardous chemicals.Results showed that the inhibition rate of seawater to Photobacteria was less than 30% in July and November,which was a low toxicity risk. The results of acute toxicity text showed that the IC_(50) of formaldehyde was6.974 mg/L, the IC_(50) of methanol was 0.048 mg/L, the IC_(50) of toluene was 0.002 mg/L, the IC_(50) of phenol was 43.484 mg/L. Moreover, the results of the acute toxicity showed that the IC_(50)value of hazardous chemicals was toluene < methanol < formaldehyde < phenol.

关 键 词：发光细菌 费歇尔弧菌 生物毒性 危化品 

分 类 号：X171.5[环境科学与工程—环境科学]