异嗜性和多变性逆转录病毒受体1在乳头状甲状腺癌患者中表达的意义  

Clinical implication of xenotropic and polytropic retrovirus receptor 1 in papillary thyroid carcinoma

作　　者：邹华伟[1] 项承 ZOU Huawei;XIANG Cheng(Department of Thyroid and Breast Surgery,Zhoukou Central Hospital,Zhoukou 466000,Henan Province,China;Department of Thyroid Surgery,the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310009,China)

机构地区：[1]河南省周口市中心医院甲状腺乳腺外科,河南周口466000 [2]浙江大学医学院附属第二医院甲状腺外科,浙江杭州310009

出　　处：《浙江大学学报（医学版）》2021年第1期90-96,共7页Journal of Zhejiang University(Medical Sciences)

摘　　要：目的:探索异嗜性和多变性逆转录病毒受体1(XPR1)基因在乳头状甲状腺癌(PTC)患者中表达的价值及其参与PTC发生发展的潜在通路。方法:通过HPA数据库和UALCAN数据库探索XPR1在PTC组织及正常组织中的表达水平;通过cBioPortal数据库获取PTC患者临床资料及XPR1基因表达值,探索XPR1表达水平与患者性别、年龄、PTC分型、T分期、N分期、M分期和疾病分期的相关性;Cox回归分析探索PTC患者预后的影响因素;利用cBioPortal数据库探索XPR1基因组学改变;基因本体(GO)和京都基因与基因组百科全书(KEGG)分析探索XPR1参与PTC的生物学途径。结果:HPA数据库分析发现,与正常组织相比,XPR1在PTC组织中表达增加;UALCAN数据库分析发现,XPR1基因表达量在PTC组织中较正常组织明显增多(P<0.01),其中高柱亚型表达量最高,滤泡亚型表达量最低。PTC患者的XPR1基因表达水平与患者年龄、PTC分型、T分期、N分期和疾病分期相关(P<0.05或P<0.01),而与性别和M分期不相关(均P>0.05)。回归分析结果显示,XPR1表达水平可作为PTC患者预后的独立影响因子(HR=2.894,P<0.05)。cBioPortal数据库显示,6%的PTC患者出现XPR1基因突变,主要表现为错义突变,突变位点为蛋白结构域E615K。富集分析结果显示,XPR1可能通过参与调节代谢通路影响PTC发生发展。结论:XPR1在PTC组织中表达增加,且影响患者预后,可能通过参与代谢通路影响PTC的发生发展,可作为PTC诊断和治疗的潜在靶点。Objective:To investigate the expression of xenotropic and polytropic retrovirus receptor 1(XPR1)in papillary thyroid cancer(PTC)and its clinical implication.Methods:The HPA and UALCAN databases were used to explore the expression of XPR1 in PTC and normal tissues.The cBioPortal database was used to obtain the clinical data of PTC patients and XPR1 gene expression profile.The correlation of XPR1 expression with gender,age,sub-types,T stage,N stage,M stage and clinical stage of patients were analyzed.Cox regression was conducted to analysis the factors affecting the prognosis of PTC patients.The mutation of XPR1 was assessed through cBioPortal database.GO and KEGG analyses were used to explore the related biological pathway of XPR1 involved in PTC.Results:HPA database analysis showed that XPR1 was highly expressed in PTC tissue compared with normal tissues.UALCAN analysis displayed that XPR1 expression was significantly higher in PTC tissue compared with normal tissues(P<0.01),and the highest and lowest expressions of XPR1 were observed in tall cell and follicular sub-type of PTC,respectively.The expression of XPR1 was correlated with age,sub-types,T stage,N stage and disease stage of PTC patients(P<0.05 or P<0.01),but was not correlated with gender and M stage(all P>0.05).Cox regression analysis showed that XPR1 was an independent prognostic factor of PTC patients(HR=2.894,P<0.05).The cBioPortal database indicated that the XPR1 mutation appeared in 6%PTC patients;the mutation type mainly was missense and the mutation point was located at the E615K.Enrichment analysis indicated that XPR1 might affect the PTC progression through involvement in metabolic pathway.Conclusions:XPR1 is highly expressed in PTC tissues,which is associated with the prognosis of patients.Metabolic pathway associated with XPR1 might play an important role in PTC progression,indicating that XPR1 might be a novel biomarker for diagnosis and treatment of PTC.

关 键 词：乳头状甲状腺癌 异嗜性和多变性逆转录病毒受体1 预后 富集分析 生物信息学分析 

分 类 号：R736.1[医药卫生—肿瘤]