壳聚糖酶的分子结构与作用机制的研究进展  

Advances in Studies on Molecular Structure and Mechanism of Chitosanase

作　　者：李振梅[1] 侯庆爱[1] 牛其东[1] 陈营[2] 温红玲 LI Zhen-mei;HOU Qing-ai;NIU Qi-dong;CHEN Ying;WEN Hong-ling(Shandong Technical Institute,Jinan 250200,China;School of Life Sciences,Yantai University,Yantai 264005,China;Department of Microbiological Laboratory Technology,School of Public Health,Shandong University,Jinan 250012,China)

机构地区：[1]山东技师学院,济南250200 [2]烟台大学生命科学学院,烟台264005 [3]山东大学公共卫生学院微生物检验学系,济南250012

出　　处：《齐鲁工业大学学报》2021年第2期34-40,共7页Journal of Qilu University of Technology

基　　金：山东省技工教育和职业培训科研课题(RSJY2017-058)。

摘　　要：壳聚糖是由N-乙酰-α-氨基-D-葡萄糖胺以β(1→4)糖苷键连结而成的线性水不溶性多糖。壳聚糖酶是水解部分乙酰化壳聚糖产生寡聚糖终产物的酶。为了提高酶法生产效率,研究人员对壳聚糖酶进行了深入的研究。本文主要对壳聚糖酶(含外切壳聚糖酶)的种类、酶的晶体结构、酶与壳聚糖的作用机制、酶的结合结构域的结构和功能、酶解特点进行综述,揭示了酶的壳聚糖结合结构域的特征,不同壳聚糖酶如何作用于壳聚糖生产活性寡聚糖。本文为筛选和改造壳聚糖酶奠定基础,对于提高酶效具有重要的意义。Chitin is a linear insoluble homo-polymer ofβ-1,4 N-acetylglucosamine(GlcNAc).Chitosanases are glucoside hydrolases that degrade the partially N-acetylated chitosan to chitosan oligosaccharides.In order to improve the enzymatic production,a number of researches have focused on chitosanases.Here the current knowledge is reviewed on the classification of chitosanases,crystal structure of chitosanases,interaction mechanism between chitosanases and chitin,the structure and function of structural elements in chitosanases and properties of enzymatic processes.And the study reveals characteristics of chitosan-binding modules and the production of chitosan oligomers through using chitosanases.The detailed insight gained here will help to select and modify chitosanases,and is of great importance to improve the catalytic efficiency.

关 键 词：壳聚糖 壳聚糖酶 晶体结构 相互作用 糖结合结构域(CBMs) 

分 类 号：TS2[轻工技术与工程—食品科学与工程]