基于Box-Behnken响应面法优化莱菔子炒制工艺及对离体十二指肠运动的影响  

Box-Behnken response surface method-based stir-frying process optimization and effects on in vitro duodenal movement for Raphanus sativus

作　　者：庞荔丹 魏娇娇 苗壮 王安君 吴家劲 薛明真 朱立俏[1] PANG Li-dan;WEI Jiao-jiao;MIAO Zhuang;WANG An-jun;WU Jia-jin;XUE Ming-zhen;ZHU Li-qiao(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《中成药》2021年第4期959-965,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81503252);山东省自然科学基金项目(ZR2020MH373);山东省本科SRT项目资助(201910441004)。

摘　　要：目的优选炒药机炒制莱菔子的最佳炒制工艺,并观察其对大鼠离体十二指肠运动的影响。方法以萝卜苷、芥子碱硫氰酸盐的含量和RS01相对含量为指标,在单因素试验的基础上,采用Box-Behnken响应面法考察炒制温度、炒制时间和炒药机转速对莱菔子炒制工艺的影响,优选出最佳工艺参数。采用MP160多导生理记录仪,观察炒莱菔子对大鼠离体十二指肠运动的影响。结果莱菔子最佳炒制工艺为炒制温度217.14℃,炒制时间3.05 min,炒制转速29.73 r/min。考虑到实际生产中的可行性,对炒制工艺调整为炒制温度220℃,炒制时间3 min,炒制转速30 r/min。炒莱菔子水提液可增大大鼠离体十二指肠收缩的张力和振幅。结论优选的莱菔子炒制工艺简单可行,更适合工业化生产的要求。莱菔子具有促进胃肠动力的作用。AIM To optimize the stir-frying process of Raphanus sativus L.,and to observe its effect on rat duodenal movement in vitro.METHODS Using the contents of glucoraphenin,sinapinethiocyanate and relative content of RS01 as indexes,and the stir-frying temperature and time,and the rotation speed of herbal medicine roaster as assessment factors,the stir-frying process of R.sativus were investigated by Box-Behnken response surface method based on single factor study.The effect on duodenal contractile activity in vitro was detected by MP160 multi-channel physiological recorder.RESULTS The ideal stir-frying process for R.sativus was 3.05 min,exposure to 217.14℃stir-frying at 29.73 r/min.Considering the feasibility in practical application,the technology was adjusted to be 3 min stir-frying at 220℃at a rotation speed of 30 r/min.The water extract of R.sativus achieved under the optimal processing conditions increased the duodenal tension and amplitude of rats in vitro.CONCLUSION The optimal stir-frying process of R.sativus is simple and feasible for the industrial production of R.sativus ensuring a promoted gastrointestinal motility.

关 键 词：莱菔子 炒制工艺 响应面法 萝卜苷 离体十二指肠 

分 类 号：R283.1[医药卫生—中药学]