猪乳中两种蛋白质的质谱鉴定  

Identification of two milk proteins of sow by mass spectrometry

作　　者：越安琪 罗卉卉 黄卫平 陈登权 金素钰[1] 黄林[1] 郑玉才[1] YUE An-qi;LUO Hui-hui;HUANG Wei-ping;CHEN Deng-quan;JIN Su-yu;HUANG Lin;ZHENG Yu-cai(College of Life Science and Technology,Southwest Minzu University,Chengdu 610041,China;Liangshan Animal Husbandry and Veterinary Research Institute,Xichang 615400,Sichuan,China)

机构地区：[1]西南民族大学生命科学与技术学院,成都610041 [2]四川省凉山州畜牧兽医研究所,四川西昌615400

出　　处：《湖北农业科学》2021年第7期103-105,共3页Hubei Agricultural Sciences

基　　金：国家级大学生创新创业项目(201810656106)。

摘　　要：为鉴定猪乳中的蛋白质组分,研究采集了8头大白猪的全乳,经离心脱脂后,采用SDS-PAGE对脱脂乳中的蛋白质进行分离,然后切取凝胶上的3条蛋白质条带,经还原烷基化和胰蛋白酶解处理后,采用液相色谱-串联质谱分析产生的水解肽,以鉴定这3种低分子量蛋白质。结果表明,获得鉴定的2种蛋白质包括β-乳球蛋白(β-LG)和乳清酸蛋白(WAP),其中WAP可能对猪乳的生物学功能有重要价值。In order to identify several protein components of porcine milk,whole milk samples of large white pigs(n=8)were collected,and skim milk was obtained by centrifugation.SDS-PAGE was employed to separate skim milk proteins,and tree protein bands on SDS-gel were cut off,followed by treatments of reductive alkylation and trypsin digestion.The resulting peptides were loaded on LCMS/MS to identify the three proteins with low molecular weight.Two proteins includingβ-lactoglobulin(β-LG)and whey acidic protein(WAP)were identified successfully.WAP may probably affect porcine milk functions.

关 键 词：猪 乳 蛋白质鉴定 质谱 

分 类 号：S828[农业科学—畜牧学]