微小RNA-513c-5p在宫颈癌中的表达及靶向组蛋白去乙酰化酶1调节宫颈癌细胞迁移和侵袭的机制  

MicroRNA-513c-5p expression in cervical cancer and targeting histone deacetylase 1 regulating cervical cancer cell migration and invasion

作　　者：王丽娜[1] 刘永娟 张莹[1] 刘荣霞[1] 梁珊[1] 宋春红[2] 崔娜[3] WANG Li-na;LIU Yong-juan;ZHANG Ying;LIU Rong-xia;LIANG Shan;SONG Chun-hong;CUI Na(Department of Gynecology,the Fourth Hospital of Shijiazhuang City,Shijiazhuang 050011,China;Department of Pathology,the Fourth hospital of Shijiazhuang City,Shijiazhuang 050011,China;Department of Obstetrics,the Second Hospital of Hebei Medical University,Shijiazhuang 050005,China)

机构地区：[1]河北省石家庄市第四医院妇科,石家庄050011 [2]河北省石家庄市第四医院病理科,石家庄050011 [3]河北医科大学第二医院妇产科,石家庄050005

出　　处：《解剖学报》2021年第1期73-77,共5页Acta Anatomica Sinica

基　　金：河北省自然科学基金(H201906674)。

摘　　要：目的探讨微小RNA(miR)-513c-5p在宫颈癌中的表达及靶向组蛋白去乙酰化酶1(HDAC1)调节宫颈癌细胞迁移和侵袭的机制。方法临床收集宫颈癌患者86例,通过Real-time PCR检测肿瘤组织和癌旁组织中miR-513c-5p水平,分析其与宫颈癌病理特征的关系。通过双荧光素酶报告验证miR-513c-5p靶向HDAC1。将宫颈癌HeLa细胞系分为4组:对照组、类似物(mimic)组、mimic+HDAC1组和HDAC1组。通过质粒转染技术过表达miR-513c-5p和(或)HDAC1。Real-time PCR和Western blotting分别用于检测RNA或蛋白的表达水平。分别通过CCK-8法、细胞划痕实验和Transwell实验检测各组的细胞生长、迁移和侵袭能力。结果宫颈癌组织中miR-513c-5p水平显著低于癌旁组织。低水平的miR-513c-5p与更高的局部侵袭、淋巴转移和远端转移有关(P<0.05)。miR-513-5p靶向抑制HDAC1表达。过表达miR-513c-5p显著抑制宫颈癌细胞生长、迁移和侵袭(P<0.05)。过表达HDAC1促进细胞生长、迁移和侵袭(P<0.05),并且可以逆转miR-513c-5p的抑制作用(P<0.05)。结论低水平的miR-513c-5p可能与宫颈癌转移有关,并且miR-513c-5p可通过靶向抑制HDAC1蛋白的表达抑制宫颈癌HeLa细胞生长、迁移和侵袭。Objective To investigate the expression of microRNA(miR)-513c-5p in cervical cancer and the mechanism of targeting histone deacetylase 1(HDAC1)regulating cervical cancer cell migration and invasion.Methods Clinically collected 86 patients with cervical cancer.The levels of miR-513c-5p in tumor tissues and adjacent tissues were detected by Real-time PCR.The relationship between miR-513c-5p and pathological characteristics of cervical cancer was analyzed.It was verified that miR-513c-5p targets HDAC1 by a dual luciferase report.Cervical cancer He La cells were divided into four groups:control group,mimic group,mimic+HDAC1 group and HDAC1 group.MiR-513c-5p and(or)HDAC1 were overexpressed by plasmid transfection technology.Real-time PCR and Western blotting were used to detect the expression level of RNA or protein,respectively.The cell growth,migration,and invasion capabilities of each group were measured by CCK-8 method,cell scratch test,and Transwell test.Results The level of miR-513c-5p in cervical cancer tissues was significantly lower than that in adjacent tissues.Low levels of miR-513c-5p were associated with higher local invasion,lymphatic metastasis,and distal metastasis(P<0.05).MiR-513-5 p targeted HDAC1 expression.Overexpression of miR-513c-5p inhibited significantly the growth,migration and invasion of cervical cancer cells(P<0.05).Overexpression of HDAC1 promoted growth,migration and invasion(P<0.05),and reversed the inhibitory effect of miR-513c-5p(P<0.05).Conclusion Low levels of miR-513c-5p might be related to cervical cancer metastasis,and miR-513c-5p could inhibit the growth,migration and invasion of cervical cancer He La cells by targeted inhibition of HDAC1 protein expression.

关 键 词：宫颈癌 微小RNA-513c-5p 组蛋白去乙酰化酶1 迁移 侵袭 双荧光素酶报告 实时定量聚合酶链反应 免疫印迹法 人 

分 类 号：R737.33[医药卫生—肿瘤]