鼻咽癌组织中JAM-A表达及意义  

Expression of JAM-A in Nasopharyngeal Carcinoma and Its Significance

作　　者：江雪[1] 代保强[2] 冯丽春[2] JIANG Xue;DAI Bao-qiang;FENG Li-chun(Cangzhou Central Hospital,Cangzhou 061000,China)

机构地区：[1]沧州市中心医院耳鼻喉科,河北沧州061000 [2]沧州市中心医院,河北沧州061000

出　　处：《肿瘤学杂志》2021年第2期125-129,共5页Journal of Chinese Oncology

基　　金：沧州市重点研发计划指导项目(183302105)。

摘　　要：[目的]探讨鼻咽癌组织中人交界粘连分子(junctional adhesion molecule,JAM-A)蛋白表达及其意义。[方法]收集100例鼻咽癌病例标本(实验组)及50例鼻咽部黏膜增生息肉标本(对照组)。采用免疫组织化学法染色测定鼻咽癌组织中JAM-A蛋白表达;采用荧光定量PCR法检测JAM-A mRNA表达;采用Western Bolot实验检测JAM-A蛋白表达。[结果]实验组JAM-A阳性表达率(63.00%)高于对照组(12.00%)(P<0.001)。不同性别、年龄、T分期、临床分期、分化程度鼻咽癌患者间JAM-A阳性表达率差异无统计学意义(P均>0.05);有淋巴结转移者JAM-A阳性表达率高于无淋巴结转移者(P=0.003)。实验组JAM-A mRNA表达量高于对照组(P<0.001);不同性别、年龄、T分期、临床分期和分化程度患者间JAM-A mRNA表达量差异无统计学意义(P均>0.05),有淋巴结转移者JAM-A mRNA表达量高于无淋巴结转移者(P<0.001)。实验组JAM-A蛋白表达灰度值高于对照组,且差异有统计学意义(P<0.05)。[结论]鼻咽癌组织中JAM-A蛋白呈高表达,且其表达与淋巴结转移相关。[Objective]To investigate the expression of junctional adhesion molecule(JAM-A)protein in nasopharyngeal carcinoma(NPC)and its significance.[Methods]One hundred NPC tissue specimens(NPC group)and 50 specimens of nasopharyngeal mucosal hyperplasia polyps(control group)were collected.The expression of JAM-A protein in tissue specimens was detected by immunohistochemical staining.The expression JAM-A mRNA and protein was detected by fluorescence quantitative PCR(qRT-PCR)and Western blot,respectively.[Results]The positive expression rate of JAM-A in NPC group was significantly higher than that in the control group(63.00%vs 12.00%,P<0.001).There was no significant difference in JAM-A positive expression rate among NPC patients with different gender,age,T stage and differentiation degree(all P>0.05).JAM-A positive rate in patients with lymph node metastasis was higher than that in patients without metastasis(P=0.003).The expression of JAM-A mRNA in the NPC group was higher than that in the control group(P<0.001).The JAM-A mRNA expression was not correlated with gender,age,T stage,clinical stage and differentiation degree of NPC patients(all P>0.05).JAM-A m RNA expression in patients with lymph node metastasis was higher than that in patients without metastasis(P<0.001).The gray value of JAM-A protein expression in the NPC group was higher than that in the control group(P<0.05).[Conclusion]JAM-A protein is highly expressed in nasopharyngeal carcinoma tissues,which is closely related to lymph node metastasis.

关 键 词：鼻咽肿瘤 JAM-A蛋白 淋巴结转移 

分 类 号：R739.63[医药卫生—肿瘤]