Fg12 ribonuclease secretion contributes to Fusarium graminearum virulence and induces plant cell death  

作　　者：Bo Yang Yuyin Wang Mengjun Tian Kaixin Dai Wenyue Zheng Zehan Liu Sen Yang Xinyu Liu Dongya Shi Haifeng Zhang Yan Wang Wenwu Ye Yuanchao Wang 

机构地区：[1]Department of Plant Pathology,Nanjing Agricultural University,Nanjing 210095,China [2]The Key Laboratory of Plant Immunity,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Journal of Integrative Plant Biology》2021年第2期365-377,共13页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31721004);the National Postdoctoral Program for Innovative Talents (BX20180142);the China Postdoctoral Science Foundation (2018M640496);the Natural Science Foundation of Jiangsu Province (BK20190520);the Fundamental Research Funds for the Central Universities (KYXK202010)。

摘　　要：Filamentous fungal pathogens secrete effectors that modulate host immunity and facilitate infection. Fusarium graminearum is an important plant pathogen responsible for various devastating diseases. However, little is known about the function of effector proteins secreted by F. graminearum. Herein, we identified several effector candidates in the F. graminearum secretome. Among them, the secreted ribonuclease Fg12 was highly upregulated during the early stages of F. graminearum infection in soybean;its deletion compromised the virulence of F. graminearum. Transient expression of Fg12 in Nicotiana benthamiana induced cell death in a light-dependent manner. Fg12 possessed ribonuclease(RNase) activity, degrading total RNA. The enzymatic activity of Fg12 was required for its cell death-promoting effects. Importantly, the ability of Fg12 to induce cell death was independent of BAK1/SOBIR1, and treatment of soybean with recombinant Fg12 protein induced resistance to various pathogens, including F. graminearum and Phytophthora sojae. Overall, our results provide evidence that RNase effectors not only contribute to pathogen virulence but also induce plant cell death.

关 键 词：cell death EFFECTOR fungal ribonuclease Fusarium graminearum VIRULENCE 

分 类 号：S432.44[农业科学—植物病理学]