miR-204-5p抑制乳腺癌细胞增殖、迁移、侵袭及对上皮-间质转化的影响  

miR-204-5p inhibits breast cancer cell proliferation, migration, invasion and its effect on epithelial-mesenchymal transition

作　　者：丁红光[1] 张星霖[2] 孙福生[3] 滑晓阳 于辉昌 DING Hong-guang;ZHANG Xing-lin;SUN Fu-sheng;HUA Xiao-yang;YU Hui-chang(Department of General Surgery,Qingdao Municipal Hospital,Qingdao 266011;Department of Oncology,Qingdao Municipal Hospital,Qingdao 266011;Department of Pharmacy,Qingdao Municipal Hospital,Qingdao 266011;Department of General Surgery,Qingdao Ninth People’s Hospital,Qingdao 266005,China)

机构地区：[1]青岛市市立医院普外一科,山东青岛266011 [2]青岛市市立医院肿瘤二科,山东青岛266011 [3]青岛市市立医院药学部,山东青岛266011 [4]青岛市市立医院普外科,山东青岛266011 [5]青岛市第九人民医院普外科,山东青岛266005

出　　处：《解剖科学进展》2021年第1期83-86,共4页Progress of Anatomical Sciences

基　　金：北京市希思科临床肿瘤学研究基金(Y-HR2015-056)。

摘　　要：目的探索miRNA-204-5p在乳腺癌组织和细胞中的表达,探讨miR-204-5p对乳腺癌细胞增殖、迁移、侵袭和上皮-间质转化(EMT)的影响。方法 qRT-PCR检测乳腺癌患者癌和癌旁组织,乳腺癌细胞和正常乳腺上皮细胞中miR-204-5p的表达;将miR-204-5p模拟物和阴性对照分别转染MCF-7细胞,并检测转染效率;CCK-8法检测转染对细胞增殖的影响;Transwell迁移实验和侵袭实验分别检测转染miR-204-5p后乳腺癌细胞迁移和侵袭能力的变化;Western blot检测上皮-间质转化(EMT)相关蛋白的表达。结果 miR-204-5p在乳腺癌组织表达水平显著下调,miR-204-5p在多种乳腺癌细胞株如MCF-7细胞表达量下调。miR-204-5p模拟物转染抑制MCF-7细胞增殖、迁移、细胞侵袭,E-cadhesin表达水平升高,Vimentin降低。结论过表达MiR-204-5p对乳腺癌有潜在的治疗作用。Objective To study the expression of miRNA-204-5 p in breast cancer tissues and cells to explore the effect of miR-204-5p on breast cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition(EMT). Methods RT-PCR was used to detect the expression of miR-204-5p mRNA;miR-204-5p mimics was transfected into MCF-7 cells, CCK-8 kid was used to detect the cells proliferation, Transwell was used to determine the cells migration and invasion, the expression of interstitial transformation(EMT) related proteins were detected by Western blot. Results The expression level of miR-204-5 p was significantly down-regulated in breast cancer tissues and breast cancer cell lines such as MCF-7 cells;miR-204-5p mimics inhibits the proliferation, migration and invasion of MCF-7 cells, increased E-cadhesin expression, but decreased Vimentin expression level. Conclusion MiR-204-5p has potential therapeutic effect on breast cancer.

关 键 词：miR-204-5p 乳腺癌 侵袭 上皮-间质转化 

分 类 号：R737.9[医药卫生—肿瘤]