肾癌免疫细胞浸润模式与生存预后分析  

Analysis of immune cell infiltration pattern and survival prognosis of renal carcinoma

作　　者：华金骏[1] 李兴[2] 王杰[1] HUA Jinjun;LI Xing;WANG Jie(Department of Urology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201210,China;Department of Anesthesiology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201210,China)

机构地区：[1]上海中医药大学附属曙光医院泌尿外科,上海201210 [2]上海中医药大学附属曙光医院麻醉科,上海201210

出　　处：《国际检验医学杂志》2021年第8期902-906,共5页International Journal of Laboratory Medicine

基　　金：国家自然科学基金面上项目(81973652)。

摘　　要：目的探讨肾癌免疫细胞浸润模式,分析浸润模式与生存预后的相关性,预测患者生存期及肿瘤进展。方法在肿瘤癌症基因图谱(TCGA)中下载肾癌转录本数据和临床信息数据,运用开源数据包CIBERSORT反卷积算法计算肾癌22种免疫细胞浸润比例。采用R软件包绘制免疫细胞相关性热图。利用R软件包对肾癌免疫细胞浸润模式与肾癌临床分期进行分析。运用Log-Rank法评估肾癌免疫细胞浸润模式与生存预后之间的关系。结果通过TCGA下载肾癌免疫细胞浸润数据,经过CIBERSORT运算和P值过滤后,得到10例正常组织免疫细胞浸润分布信息和558例肾癌组织免疫细胞浸润分布信息。在TCGA下载943例肾癌患者临床数据,经过剔除筛选后获得526例肾癌患者有效临床信息。肾癌组织与正常组织相比,M2巨噬细胞水平较高(P<0.05);幼稚B细胞、浆细胞、未活化的CD4^(+)记忆T细胞、静息态树突状细胞水平较低(P<0.05)。随着肾癌进展,未活化的NK细胞、单核细胞、未活化的肥大细胞和未活化的CD4^(+)记忆T细胞水平逐渐降低;M0巨噬细胞、滤泡辅助性T细胞、CD8^(+)T细胞、活化的CD4^(+)记忆T细胞、浆细胞和调节性T细胞水平逐渐增加。根据生存分析发现,低水平调节性T细胞、滤泡辅助性T细胞的患者5年生存率更高;高水平单核细胞、静息态树突状细胞、未活化的肥大细胞的患者5年生存率更高。结论肾癌肿瘤微环境中存在免疫细胞不同程度浸润,免疫细胞浸润模式与肾癌生存、临床分期进展密切相关,未来可以根据免疫细胞浸润模式开发出更好的靶向治疗药物。Objective To explore the infiltration pattern of immune cells in renal carcinoma,and to analyze the correlation between infiltration pattern and survival and prognosis,as well as to predict the survival time and tumor progression.Methods Transcript data and clinical information data of renal carcinoma were downloaded from The Cancer Genome Atlas(TCGA).CIBERSORT deconvolution algorithm was used to calculate the infiltration ratio of 22 kinds of immune cells in renal carcinoma.The R package was used to draw the correlation heat map of immune cells.R package was used to analyze the pattern of immune cell infiltration and staging of renal carcinoma.The Log-Rank method was used to evaluate the relationship between the infiltration pattern of immune infiltrating cells and survival prognosis of renal carcinoma.Results The data of immunocyte infiltration in renal carcinoma was downloaded by TCGA.After CIBERSORT operation and P-value filtering,the information of immunocyte infiltration distribution in 10 normal cases and 558 cases of renal carcinoma was obtained.A total of 943 cases of renal carcinoma clinical data were downloaded from TCGA website,and 526 cases of effective renal carcinoma clinical information were obtained after screening.Compared with normal tissues,level of macrophages M2 was higher in renal carcinoma tissues(P<0.05).Levels of immature B cells,plasma cells,resting CD4^(+)memory T cells,resting dendritic cells were lower(P<0.05).With the progression of renal carcinoma,the levels of resting NK cells,monocytes,resting mast cells and resting memory CD4^(+)T cells decreased gradually.M0 macrophages,follicular helper T cells,CD8^(+)T cells,activated memory CD4^(+)T cells,plasma cells and regulatory T cells increased gradually.According to survival analysis,patients with low expression of regulatory T cells and follicular helper T cells had higher 5-year survival rates.Patients with higher level of monocytes,resting dendritic cells and mast cells resting had higher 5-year survival rates.Conclusion There are

关 键 词：肾癌 免疫细胞浸润模式 生存预后 临床分期 

分 类 号：R737.11[医药卫生—肿瘤]