滑动弧放电等离子体对枯草芽孢杆菌的灭菌机理  

Sterilization Mechanism of Bacillus subtilis by Gliding Arc Discharge Plasma

作　　者：崔鲁彬 孙运金[1,2] 王雪莹 王艳 CUI Lubin;SUN Yunjin;WANG Xueying;WANG Yan(Beijing University of Agriculture,Beijing 102206,China;Plasma Engineering Center,Capital Agricultural Product Safety Industrial Technology Institute,Beijing University of Agriculture,Beijing 102206,China;Chinese Academy of Agricultural Mechanization Sciences,Beijing 100083,China)

机构地区：[1]北京农学院,北京102206 [2]北京农学院首都农产品安全产业技术研究院等离子体工程中心,北京102206 [3]中国农业机械化科学研究院,北京100083

出　　处：《农业工程》2021年第1期62-68,共7页AGRICULTURAL ENGINEERING

基　　金：北京农学院学位与研究生教育改革与发展项目(项目编号:5076516017∕026)。

摘　　要：滑动弧放电是在大气环境下产生的一种等离子体形式,放电产生的活性粒子对环境中存在的各种食源性有害微生物具有较好的抑菌效果。以枯草芽孢杆菌为处理对象,研究了放电处理时间对枯草芽孢杆菌的杀菌效果,并通过表面形貌、电导率和共聚焦显微镜等测试方法揭示滑动弧放电等离子体的失活机制。结果表明,氮气放电等离子体具有较好的杀菌效果,主要抑菌机理是对细菌的细胞膜造成破坏,导致细胞坏死。Gliding arc discharge plasma is a kind of discharge phenomenon in atmospheric environment, during which active particles have good bacteriostatic effect on various foodborne harmful microorganisms in environment. Sterilization effect of discharge treatment time against bacillus subtilis was studied and inactivation mechanism of gliding arc discharge plasma was revealed by surface morphology, electrical conductivity and confocal microscopy by taking bacillus subtilis as treated samples. Results showed that nitrogen discharge plasma had better bactericidal effect, whose main bacteriostatic mechanism was to destroy cell membrane of bacteria, leading to cell necrosis.

关 键 词：滑动弧放电等离子体 表面形貌 灭菌机理 细胞坏死 

分 类 号：TS201.3[轻工技术与工程—食品科学]