灵芝液体浅层静置培养高效生产三萜的研究  

An efficient strategy to enhance triterpenoid production by liquid superficial-static culture(LSSC)of Ganoderma lingzhi

作　　者：王琼 徐萌萌 刘高强[3] 曹春蕾 石贵阳[1,2] 丁重阳 WANG Qiong;XU Meng-Meng;LIU Gao-Qiang;CAO Chun-Lei;SHI Gui-Yang;DING Zhong-Yang(Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi,Jiangsu 214122,China;National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi,Jiangsu 214122,China;Hunan Provincial Key Laboratory for Forestry Biotechnology&International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology,Central South University of Forestry and Technology,Changsha,Hunan 410004,China)

机构地区：[1]江南大学生物工程学院糖化学与生物技术教育部重点实验,江苏无锡214122 [2]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122 [3]中南林业科技大学森林资源生物技术湖南省国际科技创新合作基地(国际联合实验室),湖南长沙410004

出　　处：《菌物学报》2021年第3期656-667,共12页Mycosystema

基　　金：Supported by the National Natural Science Foundation of China(31271918);Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX181787);National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-22)。

摘　　要：三萜是灵芝Ganoderma lingzhi中重要的活性物质。本研究采用液体浅层静置培养方式(LSSC)提高灵芝三萜的产量。结果表明,在T25细胞培养瓶中的最佳培养条件为初始培养体积2mL,接种量7.0g(菌体湿重/L),在48h补加2mL培养液,发酵7d,三萜产量可达(32.95±0.51)mg/g,为摇瓶培养最高产量的2.09倍。进一步经不锈钢平盘放大研究发现,三萜最高产量为(65.91±0.84)mg/g,为摇瓶培养的4.19倍。形态学研究表明,浅层静置培养过程中形成的气生菌丝是提高三萜产量的关键因素。与深层液体发酵相比,液体浅层静置培养具有生长快,三萜产量高,发酵过程不需要搅拌等优点,具有良好的工业化应用前景。Triterpenoid was recognized as the major bioactive molecule of Ganoderma lingzhi.In this work,liquid superficial-static culture(LSSC)process was used to enhance the production of triterpenoid.After optimizing,the best condition to produce triterpenoid in LSSC was initial medium volume 2 mL,inoculum size 7.0 g/L,adding medium 2 mL in 48 h and fermentative duration 7 d.Under the optimal conditions,the triterpenoid production scaled(65.91±0.84)mg/g,being 4.19-fold that in submerged culture.The results demonstrated that aerial mycelial formation was a key factor for triterpenoid biosynthesis of G.lingzhi.Compared with submerged culture,LSSC exhibited growing fast and high triterpenoid production and productivity needless to stir or shake,and had potential for industrial application.

关 键 词：灵芝 液体浅层静置培养 三萜 气生菌丝 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]