石墨烯量子点提高DNAzyme在细胞水平上的稳定性  

Graphene quantum dots improve the stability of DNAzyme in cells

作　　者：黄奇萍 刘慧 张井岩 郑静 HUANG Qiping;LIU Hui;ZHANG Jingyan;ZHENG Jing(State-Key Laboratory of Bioreactor Engineering;School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室 [2]华东理工大学药学院,上海200237

出　　处：《生物学杂志》2021年第2期26-31,共6页Journal of Biology

基　　金：国家自然科学基金(21671065);中央高校基本科研业务费专项资金。

摘　　要：为了提高脱氧核糖核酸酶(又称DNAzyme)的稳定性,以石墨烯量子点(GQDs)为载体,负载两个靶向TNFzyme与GQDs结合后,不仅保持了原有的DNAzyme活性,而且稳定性得到了提高。结果初步表明GQDs可以作为50%,而单独DNAzyme作用的细胞中TNF-α蛋白表达量则基本不变,这表明GQDs可以将该DNAzyme载入细胞内,的作用而使它在细胞水平的稳定性得到改善。In this work,we employed Graphene quantum dots(GQDs)as a carrier loading two RNA-cleaving Deoxyribonuclease(DNAzymes)to explore their activities at molecular and cellular levels.The two DNAzymes were designed and synthesized based on the mRNA of TNF-α(TNF-DNAzyme1 and TNF-DNAzyme2).We demonstrated that the TNF-DNAzymes/GQDs conjugates were stable in solution,and the catalytic activity of the DNAzymes remained in the presence of GQDs.The expression level of TNF-αin Hela cells was significantly decreased after the incubation with TNF-DNAzymes and GQDs.In contrast,the expression level of TNF-αin Hela cells was barely changed after the incubation with TNF-DNAzymes alone,which indicated that GQDs could be used as a carrier to deliver TNF-DNAzymes into cells,thus the activity of TNF-DNAzymes in cellular level could be greatly improved.Based on the control experiments,we inferred that the improved activity of DNAzymes could be due to their improved stability with GQDs.However,we could not rule out which caused by the higher accumulation of DNAzymes in the cells or other mechanisms.

关 键 词：脱氧核糖核酸酶 石墨烯量子点 稳定性 TNF-Α 

分 类 号：R914[医药卫生—药物化学] R965[医药卫生—药学]