Melanization induced by Heliothis virescens ascovirus 3h promotes viral replication  

作　　者：Zi-Qi Li Xiao-Hui Song Min Wang Shu Wang Guo-Hua Huang 

机构地区：[1]Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,Hunan Agricultural University,Changsha,China [2]College of Plant Protection,Hunan Agricultural University,Changsha,China

出　　处：《Insect Science》2021年第2期472-484,共13页昆虫科学（英文版）

基　　金：We would like to thank the American Journal Experts teamfor editing and revising the English language.This work was supported by the National Natural Science Foundation of China(31872027);Changsha Science and Technology Project(kq 1901033),and Double first-class construction project of Hunan Agricultural University.

摘　　要：Melanization is an important innate immune defense mechanism of insects,which can kill invading pathogens.Most pathogens,for their survival and reproduction,inhibit the melanization of the host.Interestingly,our results suggested that after infection with Heliothis virescens ascovirus 3h(HvAV-3h),the speed of melanization in infected Spodoptera exigiia larval hemolymph was accelerated and that the phenoloxidase(PO)activity of hemolymph in larvae infected with HvAV-3h increased significantly(1.20-fold at 96 hpi,1.52-fold at 120 hpi,1.23-fold at 144 hpi,1.12-fold at 168 hpi).The transcription level of the gene encoding S.exigua prophenoloxidase-1(SePPO-1 gene)was upregulated dramatically in the fat body during the middle stage of infection.In addition,when melanization was inhibited or promoted,the replication of HvAV-3h was inhibited or promoted,respectively.In conclusion,infection with HvAV-3h can markedly induce melanization in the middle stage of infection,and melanization is helpful for HvAV-3h viral replication.

关 键 词：Heliothis virescens ascovirus 3h MELANIZATION Spodoptera exigua viral replication 

分 类 号：S476[农业科学—农业昆虫与害虫防治]