Grainy head signaling regulates epithelium development and ecdysis in Blattella germanica  

作　　者：Zheng Zhao Liang Li Min Cheng An-Di Jing Su-Ning Liu Shi-Ming Zhu Er-Xia Du Sheng Li Yun-Xia Luan Chong-Hua Ren 

机构地区：[1]Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Institute of Insect Science and Technology,School of Life Sciences,South China Normal University,Guangzhou,China [2]Guangmeiyuan R&D Center,Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,South China Normal University,Meizhou,Guangdong Province,China

出　　处：《Insect Science》2021年第2期485-494,共10页昆虫科学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Grant No.31702055,No.31572325 and No.31702021);CR.SL and ED,by the Natural Science Foundation of Guangdong Province(Grant No.2020A1515011267);CR,by the National Key Research and Development Project(Grant No.2019YFD1002102);CR and YL,by the Department of Science and Technology in Guangdong Province(Grant No.2019B090905003 and 2019A0102006);Shenzhen Science and Technology Program(Grant No.KQTD20180411143628272);SL.The strategies for cockroach management mentioned in this work have been included in a Chinese patent application(201910977154.9;201910976989.2).

摘　　要：The transcription factor grainy head(Grh)functions in the protection of the epithelium against the external environment by generating strongly adhesive layers,and this function is conserved in vertebrates and invertebrates.In Drosophila,the top model for holometabolous insects,Grh is necessary during embryonic development,epidermal differentiation,central nervous system specification and epithelial repair.However,the function of this gene in hemimetabolous insect epithelia remains unknown.To examine the function of Grh signaling in regulating epithelium development in Hemimetabola,we focused on the Blattella germanica epidermal layer using a gene knockdown strategy.The spatiotemporal expression pattern of BgGrh was detected,and knockdown of Bg-Grh and BgCad96ca,which provide positive feedback to BgGrh,caused severe defects in new epithelium development and impeded the molting process required to discard the old integument.Knockdown of the expression of BgGrh and BgCad96ca caused increased expression of chitin synthase gene{BgCHSl)and chitinase gene{BgCht5),the upregulations of which should be mediated by the higher level of hormone receptor 3{BgHr3)gene.In conclusion,epithelium development is regulated by Grh signaling,which might represent a potential target for the control of urban pest cockroaches.

关 键 词：BgCad96ca COCKROACH ECDYSIS EPITHELIUM Grh signaling 

分 类 号：Q96[生物学—昆虫学]