谷氨酸全营养流加发酵新工艺  

New technology of total nutrient feeding fermentation of glutamic acid

作　　者：刘景阳 刘云鹏 徐庆阳[1,2,3] LIU Jingyang;LIU Yunpeng;XU Qingyang(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;Tianjin Engineering Lab of Efficient and Green Amino Acid Manufacture,Tianjin 300457,China;National and Local United Engineering Lab of Metabolic Control Fermentation Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津市氨基酸高效绿色制造工程实验室,天津300457 [3]代谢控制发酵技术国家地方联合工程实验室,天津300457

出　　处：《食品与发酵工业》2021年第7期14-20,共7页Food and Fermentation Industries

摘　　要：全营养流加主要是选择适当的全营养培养,在合适的时间进行营养的补加,通过补加的营养来弥补菌体因生长代谢而消耗的营养物质,同时也可以降低发酵培养基的浓度,避免富营养对于菌体活力的抑制。因此,采用全营养流加策略能够解决L-谷氨酸发酵后期菌体活力不足和产酸能力下降等问题。实验结果表明,最佳流加条件为从发酵2 h开始流加,持续24 h流加体积分数为60%的流加培养基。在此条件进行L-谷氨酸发酵,生物量(OD 600)达到了66,提升了29.4%,菌体转型时间提前了2 h,L-谷氨酸产量为168 g/L,提高了22.6%,乳酸含量为3.1 g/L,降低了13.8%,丙氨酸含量为2.06 g/L,降低了17.6%,糖酸转化率为63%,提高了1.5%。全营养流加发酵对于加快菌体转型,提高菌体活力、谷氨酸产量及糖酸转化率均有积极作用。Total nutrient feeding mainly refers to feeding a suitable total nutrient medium at an appropriate time to supplement the nutrients consumed by bacterial growth and metabolism.Adopting the strategy of total nutrient feeding can also reduce the concentration of the fermentation medium to avoid the inhibition of eutrophication to the cell viability.Therefore,adopting a total nutrient feeding strategy can solve the problems of insufficient cell viability and decreased acid production capacity in the late stage of L-glutamic acid fermentation.Through experimental analysis,it was determined that the optimal feeding condition was to start feeding with a volume fraction of 60%for 24 h after 2 h of fermentation.Under this condition,the biomass reached 66,which was increased by 29.4%,the transformation time of the bacteria was advanced by 2 h,and the L-glutamic acid production reached 168 g/L,which was an increase of 22.6%.The lactic acid content was 3.1 g/L,decreased by 13.8%;the alanine content was 2.06 g/L,with a reduction of 17.6%,and the sugar-acid conversion rate was 63%,with an increase of 1.5%.Total nutrient feeding fermentation has a positive effect on accelerating the transformation of the bacteria,improving the cell viability,increasing the production of L-glutamic acid and sugar acid conversion rate.

关 键 词：L-谷氨酸 菌体活力 全营养流加发酵 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]