口服三七总皂甙对大黄鱼免疫功能的影响  

Effects of oral Panax notoginseng saponins on immunity of large yellow croaker(Pseudosciaena crocea)

作　　者：陈秀霞[1] 池洪树[1] 许斌福[1] 谢岸桦 方冬兰[1] 龚晖[1] CHEN Xiuxia;CHI Hongshu;XU Binfu;XIE Anhua;FANG Donglan;GONG Hui(Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China)

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003

出　　处：《浙江农业学报》2021年第4期610-617,共8页Acta Agriculturae Zhejiangensis

基　　金：福建省属公益类科研院所专项(2018R1019-6);福建省农业科学院项目(AC2017-1)。

摘　　要：将含不同浓度三七总皂甙(PNS)的饲料(0、10、50、250 mg·kg-1)连续14 d投喂大黄鱼,检测肝、脾、头肾组织部分免疫相关基因的表达,以及血清部分免疫因子的活性,结合人工注射感染变形假单胞菌的抵抗力,评估口服PNS对大黄鱼免疫功能的影响。实验结果显示,各浓度组大黄鱼血清超氧化物歧化酶(SOD)、溶菌酶(LZM)、酸性磷酸酶(ACP)和碱性磷酸酶(ALP)活性与对照组相比无显著差异;高浓度组(250 mg·kg-1)大黄鱼脾组织粘病毒抗性蛋白(Mx)的相对表达水平显著高于对照组,中浓度组(50 mg·kg-1)脾组织和低浓度组(10 mg·kg-1)头肾组织的Mx相对表达水平略高于对照组,但差异不显著;各浓度组各组织肿瘤坏死因子α链(TNF-α)的相对表达水平与对照组无显著差异,但均低于对照组;各浓度组各组织中白细胞介素10(IL-10)和主要组织相容性复合体Ⅱ类α链(MHCⅡ-α)的相对表达水平与对照组无显著差异。感染变形假单胞菌后各浓度组大黄鱼的死亡率与对照组无显著差异。结果表明,饲料中添加10~250 mg·kg-1的PNS连续投喂14 d未能明显增强大黄鱼免疫机能。In this study,large yellow croaker(Pseudosciaena crocea)were fed with diets containing 0,10,50,250 mg·kg-1 Panax notoginseng saponins(PNS)for 14 d.To evaluate the effects of PNS on the immunity of large yellow croaker,the expression of some immune-related genes in the liver,spleen and head kidney tissues,the activities of some serum immune factors and the resistance against Pseudomonas plecoglossicida were determined.Results showed that the activities of serum superoxide dismutase(SOD),lysozyme(LZM),acp acid phosphatase(ACP)and alkaline phosphatase(AKP)in the experimental groups were not significantly different from the control group;the expression of Mx in spleen of fish fed with 250 mg·kg-1 PNS was significantly higher than that of control;the expression of Mx in spleen of fish fed with 50 mg·kg-1 PNS and in head-kidney of fish fed with 10 mg·kg-1 PNS were higher than that of control,but not significantly;the expression of TNF-αin all the exprimental groups were lower than that of control,but not significantly;the expression of IL-10 and MHCⅡ-αin all the exprimental groups were not significantly different from the control.The mortalities of the exprimental fish challenged with P.plecoglossicida were not significantly different from the control.It was indicated that feeding diets containing 10-250 mg·kg-1 PNS for 14 d did not improve the immunity of large yellow croaker.

关 键 词：三七总皂甙 大黄鱼 免疫 

分 类 号：S965.322[农业科学—水产养殖]