Application of monoclonal antibody against ginsenoside in ginseng research:a review  

作　　者：Yui Sasaki Koichi Shimizu Hitoshi Watanabe Yukihiro Shoyama 

机构地区：[1]Association for Health Economics Research and Social Insurance and Welfare,Tokyo 105-0003,Japan [2]Center for Environment,Health and Field Sciences,Chiba University,Kashiwa 277-0882,Japan [3]Faculty of Pharmacy,Nagasaki International University,Nagasaki 859-3298,Japan

出　　处：《Traditional Medicine Research》2021年第3期66-77,共12页TMR传统医学研究

摘　　要：Panax genus belonging to a family of Araliaceae grow in Asia(9 species)and in North America(2 species).Especially Panax ginseng was listed in Chinese medical book,Shennong's Classic of Materia Medica approximately 2 thousand years ago and Panax species are now one of the most important natural medicine.Since Panax species contain approximately 260 ginsenosides,its quality control and pharmacological movement of ginsenoside in body are not enough understanding.Monoclonal antibodies against ginsenosides were prepared and set up the enzyme linked immunosorbent assay system for the quality control of natural product.Furthermore,we developed Eastern blotting system using monoclonal antibodies resulted that protopanaxadiol and protopanaxatriol group ginsenosides can be separately stained by two corresponding monoclonal antibodies,respectively.It became evident that the ginseng slice was stained by Eastern blotting system.Histochemical staining of ginseng can make clear the ginsenoside-Rb1 distribution in cells and tissues.Double Eastern blotting system facilitated by two monoclonal antibodies like anti-ginsenoside-Rb1 and anti-ginsenoside-Rg1 monoclonal antibodies gives several information such as sugar number,structure,and qualitative/quantitative evaluation.Immunoaffinity column combined with monoclonal antibodies succeeded one-step isolation of ginsenoside and make it possible to prepare knockout extract of which only antigen molecule was removed suggesting the pharmacological and biological value of antigen molecule in the crude extract.

关 键 词：Panax species GINSENOSIDE Monoclonal antibody Pharmacological activity Eastern blotting Knockout extract 

分 类 号：R28[医药卫生—中药学]