黑木耳菌糠基质下毛木耳细胞工程菌株的生殖生长  

Reproductive growth of engineered strains of Auricularia polytricha cells under Auricularia auricularia bran matrix

作　　者：刘微 徐凡 杨超上 耿丹萌 张铁军 王谦[1] LIU Wei;XU Fan;YANG Chaoshang;GENG Danmeng;ZHANG Tiejun;WANG Qian(College of Life Sciences,Hebei University,Baoding 071002,China;Agricultural Environmental Protection Monitoring Station,Bureau of Agriculture and Rural Affairs of Chengde City,Chengde 067000,China)

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]承德市农业农村局农业环境保护监测站,河北承德067000

出　　处：《河北大学学报（自然科学版）》2021年第2期195-200,共6页Journal of Hebei University(Natural Science Edition)

基　　金：河北省科技厅食用菌育种专项(16227303D-2);河北省二期现代农业产业技术体系创新团队项目(HBCT2018050204)。

摘　　要：以毛木耳[Auricularia polytricha(Mont.)Sacc]细胞工程菌株新河大SL205为供试菌株,以黑木耳菌糠为主要生殖生长基料,以菌丝长速、生殖生长生物学效率为指标,设计不同培养基配方进行实验.结果表明:供试菌株新河大SL205的适宜培养基配方为黑木耳菌糠50%(质量分数,下同)、木屑30%、玉米芯10%、麸皮8%、石灰2%;该培养基下,新河大SL205平均长速为7.26 mm/d,生物学效率为126.29%,对黑木耳菌糠生物降解能力最强,有效降低了黑木耳菌糠带来的面源污染.The Auricularia polytricha(Mont.)Sacc cell engineering strain Xinheda SL205 was used as the test strain,the A.auricularia bran was used as the main reproductive growth substrate,and the mycelium growth rate and reproductive growth biological efficiency were used as indicators.Different medium formulations were designed and tested.The optimal medium formulations were selected for the Xinheda SL205 strain:A.auricularia bran is 50%(mass fraction),sawdust 30%,corn cob 10%,bran 8%and lime 2%.Under this medium,the average growth rate of Xinheda SL205 was 7.26 mm/d,and the biological efficiency was 126.29%.It had the strongest biodegradation ability for A.auricularia bran,effectively reducing the non-point source pollution caused by A.auricularia bran.

关 键 词：毛木耳 黑木耳菌糠 资源化开发 生殖生长 生物转化率 

分 类 号：S646[农业科学—蔬菜学]