黑牛肝菌多糖超声提取工艺优化及抗氧化研究  

Study on Optimization of Ultrasonic Extraction Process and Antioxidant of Polysaccharides from Boletus aereus

作　　者：景年华[1] 史俊友[1] 田照秀 成琴 JING Nian-hua;SHI Jun-you;TIAN Zhao-xiu(Qujing Normal University,Qujing,Yunnan 655011)

机构地区：[1]曲靖师范学院,云南曲靖655011

出　　处：《安徽农业科学》2021年第8期170-174,共5页Journal of Anhui Agricultural Sciences

基　　金：云南省科技计划项目(2017FD146)。

摘　　要：以云南大理黑牛肝菌为材料,使用超声辅助萃取及水提醇沉法提取多糖,采用单因素试验及正交试验对黑牛肝菌多糖提取工艺进行优化,并对黑牛肝菌多糖进行了提取;对所得多糖进行DPPH自由基、ABTS自由基清除能力及铁氰化钾还原能力试验,对其抗氧化活性进行比较研究。结果表明,黑牛肝菌多糖最佳提取工艺为料液比1∶25(g∶mL)、醇沉浓度80%、超声时间70 min、超声功率90%,在此条件下多糖含量为79.41 mg/g。醇沉浓度为80%时,多糖提取物抗氧化活性最强,多糖对DPPH、ABTS自由基清除率及总还原力吸光度分别为97.92%、99.80%和0.789;醇沉浓度为50%时,所得多糖对DPPH、ABTS自由基清除率及总还原力吸光度分别为85.79%、88.23%和0.713,抗氧化活性最低;表明黑牛肝菌多糖对自由基有较好的清除效果及还原作用。With Dali Boletus aereus as the material,the polysaccharide was extracted by ultrasonic-assisted extraction and water extraction and alcohol precipitation.Single factor test and orthogonal test were used to optimize the extraction process of Boletus aereus polysaccharides,and the polysaccharides of Boletus aereus were extracted.Additionally,a comparative study on scavenging abilities of DPPH,ABTS and reducing power of potassium ferricyanide for the obtained polysaccharides was carried out.The results showed that the best extraction technology of polysaccharides from Boletus aereus was the solid-liquid ratio1∶25(g∶mL),ethanol concentration of 80%,ultrasonic time of 70 min and ultrasonic power of 90%.Content of polysaccharide obtained from Boletus aereus was 79.41 mg/g under the best extraction conditions.Antioxidant activity of polysaccharide precipitated by 80%ethanol was the strongest,scavenging rates of DPPH,ABTS and absorbance of total reducing power were 97.92%,99.80%and 0.789 respectively.Antioxidant activity of polysaccharide precipitated by 50%ethanol was the lowest,scavenging rates of DPPH,ABTS and absorbance of total reducing power were 85.79%,88.23%and 0.713,respectively.These results showed that polysaccharides from Boletus aereus had good scavenging ability and reducing power.

关 键 词：黑牛肝菌 多糖 超声波辅助萃取 工艺优化 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]