HPLC法同时测定三九胃泰颗粒中8个成分含量  

Simultaneous Determination of 8 Constituents in Sanjiu Weitai Granules by HPLC

作　　者：沈丹萍 陈康 金夏萍 张洪成 章娟娟 Shen Danping;Chen Kang;Jin Xiaping;Zhang Hongcheng;Zhang Juanjuan(Jinhua Cuangfu Cancer Hospital,Zhejing Jimhua 32100,China)

机构地区：[1]金华广福肿瘤医院药剂科,浙江金华321000

出　　处：《中国药师》2021年第4期766-769,共4页China Pharmacist

摘　　要：目的:建立HPLC法同时测定三九胃泰颗粒中8个成分的含量。方法:采用HPLC法,同时测定三九胃泰颗粒中氯化两面针碱、没食子酸、梓醇、芍药苷、黄芩苷、汉黄芩苷、木香烃内酯和去氢木香烃内酯的含量,采用日本岛津GL Inert Sustain AQ C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈(A)-0.05%磷酸溶液(B),梯度洗脱;检测波长:250 nm,流速:1.0 ml·min-1,柱温:30℃,进样量:10μl。结果:氯化两面针碱、没食子酸、梓醇、芍药苷、黄芩苷、汉黄芩苷、木香烃内酯和去氢木香烃内酯检测质量浓度的线性范围分别为24.36~341.00μg·ml-1、11.25~157.60μg·ml-1、7.32~102.50μg·ml-1、36.18~506.50μg·ml-1、30.28~423.90μg·ml-1、13.05~182.60μg·ml-1、18.22~255.10μg·ml-1、8.55~119.70μg·ml-1(r为0.999 2~0.999 9);平均加样回收率分别为98.70%,98.60%,98.10%,98.90%,98.80%,98.50%,98.30%,98.10%(RSD <2.0%,n=6)。结论:该方法简单、有效、结果准确,可用于三九胃泰颗粒中上述8个成分含量的同时测定。Objective: To establish an HPLC method for the simultaneous determination of 8 constituents in Sanjiu Weitai granules. Methods: The determination was performed on a GL Inert Sustain AQ C18 column with the mobile phase consisting of acetonitrile-0.05% phosphoric acid(gradient elution) at the flow rate of 1.0 ml·min-1. The detection wavelength was set at 250 nm. The column temperature was set at 30℃ and the injection volume was 10 μl. Results: The linear range was 24.36-341.00 μg·ml-1 for nitidine chloride,11.25-157.60 μg·ml-1 for gallic acid,7.32-102.50 μg·ml-1 for catalpol,36.18-506.50 μg·ml-1 l for paeoniflorin,30.28-423.90 μg·ml-1 for baicalein,13.05-182.60 μg·ml-1 for wogonoside,18.22-255.10 μg·ml-1 for costunolide and 8.55-119.70 μg·ml-1 for dehydrocostuslactone(r = 0.999 2-0.999 9);the average recovery was 98.70%,98.60%,98.10%,98.90%,98.80%,98.50%,98.30% and 98.10%(RSD<2.0%,n = 6),respectively. The RSDs of precision,stability tests(24 h) and reproducibility were all lower than 2.0%(n = 6). Conclusion: The method is simple,effective and accurate. It can be used for the simultaneous determination of 8 constituents in Sanjiu Weitai granules.

关 键 词：高效液相色谱法 三九胃泰颗粒 氯化两面针碱 没食子酸 梓醇 芍药苷 黄芩苷 汉黄芩苷 木香烃内酯 去氢木香烃内酯 含量测定 

分 类 号：R927.2[医药卫生—药学]