CYP2E1和CYP1A1基因型与广州北部地区人群HBV易感性间的相关性分析  

The correlation between CYP2E1 and CYP1A1 genotypes with the susceptibility of HBV infection in northern Guangzhou people

作　　者：洪晓绿[1] 徐沛演 潘小平[2] HONG Xiao-lv;XU Pei-yan;PAN Xiao-ping(Department of Infection DiseasesHuadu District People′s Hospital,Guangzhou,Guangdong 510800,China;Department of Clinical Laboratory,Huadu District People′s Hospital,Guangzhou,Guangdong 510800,China)

机构地区：[1]广州市花都区人民医院感染性疾病科,510800 [2]广州市花都区人民医院检验科,510800

出　　处：《肝脏》2021年第2期131-135,共5页Chinese Hepatology

基　　金：花都区医疗卫生一般科研专项(19-HDWS-031)。

摘　　要：目的分析CYP2E1和CYP1A1基因型与广州北部地区人群HBV易感性间的相关性。方法采用SnapShot法检测150例HBV感染患者(实验组)和150名体检健康人群(对照组)CYP2E1基因rs3813867、rs2031920及CYP1A1基因rs4646421、rs2198843位点基因型别,比较两组各基因型分布频率差异;实验组同时检测ALT、AST、GGT、TBil、AFP、HBV DNA,并分析这些临床指标与有突变意义基因位点间的关系。结果两组人群符合Hardy-Weinberg平衡(均P>0.05),具备群体代表性;CYP2E1基因rs4646421和rs2198843基因型分布频率在两组间差异无统计学意义(均P>0.05),实验组CYP2E1基因rs3813867和rs4646421基因型分布频率较对照组差异有统计学意义(均P<0.05);rs3813867位点,CC基因型个体发展肝炎的风险是GG基因型个体的5.59倍,rs2031920位点,TT基因型个体发展肝炎的风险是CC基因型个体的3.57倍;CYP2E1基因rs3813867和rs4646421基因型分布频率在各临床指标间差异均无统计学意义(均P>0.05)。结论CYP1A1基因rs4646421和rs2198843位点基因分型与广州北部地区人群HBV易感性无关,CYPE1基因rs3813867和rs4646421位点基因分型与广州北部地区人群HBV易感性密切相关。Objective To analyze the association between CYP2E1 and CYP1A1 genotypes with the susceptibility of HBV infection in northern Guangzhou people.Methods 150 patients with HBV infection were enrolled as the experimental group and 150 healthy cases were selected as the control group.The polymorphisms of rs3813867 and rs2031920 in CYP2E1 gene,and rs4646421 and rs2198843 in CYP1A1 gene were detected in all people by snapshot.The frequencies of the distributions of the genotypes were compared between these two groups.Clinical data of alanine aminotransferase(ALT),aspartate aminotransferase(AST),glutamyl transpeptidase(GGT),total bilirubin(T-Bil),alpha fetoprotein(AFP)and HBV DNA were collected and analyzed for their relationship with significance mutation genotypes.Results Hardy-Weinberg equilibrium was found in both groups(P>0.05).The frequencies of rs4646421 and rs2198843 genotypes of CYP1A1 gene in experimental group were not significantly different between two groups(P>0.05),the frequencies of rs3813867 and rs4646421 genotypes of CYPE1 gene in experimental group were significantly different between two groups(P<0.05);At rs3813867 locus,the risk of hepatitis in individuals with CC genotype was 5.59 times higher than those with GG genotype.At rs2031920 locus,the risk of developing hepatitis in individuals with TT genotype was 3.57 times higher than those with CC genotype,but the frequencies of rs3813867 and rs4646421 genotypes in CYPE1 gene had no significant difference among all clinical indicators(all P>0.05).Conclusion Rs3813867 and rs4646421 of CYPE1 gene but not rs4646421 and rs2198843 in CYP1A1 gene were associated with the susceptibility of HBV infection in northern Guangzhou people.

关 键 词：rs3813867 rs2031920 rs4646421 rs2198843 基因分型 HBV 

分 类 号：R73[医药卫生—肿瘤]