miR-133b靶向HER-2对结肠癌细胞放射敏感性影响  

Effect of miR-133b on radiosensitivity of colon cancer SW620 cells by targeting HER-2

作　　者：赵磊 王天玉 王晓敏 刘俊中 易善永 Zhao Lei;Wang Tianyu;Wang Xiaomin;Liu Junzhong;Yi Shanyong(Department of Intervention,Zhengzhou Central Hospital Affiliate to Zhengzhou University,Zhengzhou 450000,China;Department of Radiation,Zhengzhou Central Hospital Affiliate to Zhengzhou University,Zhengzhou 450000,China;Depatment of Oncology,Zhengzhou Central Hospital Affiliate to Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学附属郑州中心医院介入科,450000 [2]郑州大学附属郑州中心医院放疗科,450000 [3]郑州大学附属郑州中心医院肿瘤科,450000

出　　处：《中华放射肿瘤学杂志》2021年第4期403-406,共4页Chinese Journal of Radiation Oncology

基　　金：河南省医学科技攻关计划省部共建项目(201601030)。

摘　　要：目的研究miR-133b对结肠癌细胞(SW620细胞)凋亡及放射敏感性影响并探讨其机制。方法采用脂质体法转染miR-con组(转染miR-con)、miR-133b组(转染miR-133b mimics)、si-con组(转染si-con)和si-HER-2组(转染si-HER-2)SW620细胞,然后进行0、2、4、6、8 Gy照射。使用qRT-PCR、Western blot、流式细胞术、克隆形成实验和双荧光素酶报告基因实验分别检测各组细胞中miR-133b表达、HER-2蛋白表达、细胞凋亡、细胞存活分数和细胞荧光活性。结果与照射前相比,照后SW620细胞中miR-133b表达降低(P<0.05),HER-2表达升高(P<0.05)。过表达miR-133b、敲减HER-2均可降低SW620细胞存活分数(P<0.05),促进细胞凋亡(P<0.05)。miR-133b可抑制野生型HER-2细胞荧光活性(P<0.05),且可负向调控HER-2蛋白表达。结论miR-133b可抑制SW620细胞存活,促进细胞凋亡,增强放射敏感性,其机制可能与靶向HER-2有关。Objective To evaluate the effect of miR-133b on the apoptosis and radiosensitivity of colon cancer cell line(SW620 cells),and to explore its mechanism.Methods SW620 cells were transfected with miR-con(miR-con group),miR-133b mimics(miR-133b group),si-con(si-con group)and si-HER-2(si-HER-2 group)by the liposome method,and then irradiated with 0,2,4,6,8 Gy.The miR-133b protein expression,HER-2 protein expression,apoptosis,cell survival fraction and cytofluoroactivity in each group were evaluated by qRT-PCR,Western blot,flow cytometry,colony formation assay and dual luciferase reporter gene assay,respectively.Results Compared with the pre-irradiation group,the expression level of miR-133b was significantly down-regulated(P<0.05),whereas that of HER-2 was significantly up-regulated in SW620 cells after irradiation(P<0.05).Overexpression of miR-133b and knockdown of HER-2 remarkably reduced the survival fraction(both P<0.05),and significantly promoted the apoptosis of SW620 cells(P<0.05).miR-133b could considerably inhibit the fluorescent activity of wild-type HER-2 cells(P<0.05)and negatively regulate the expression of HER-2 protein.Conclusion miR-133b can inhibit the survival of colon cancer cells,promote the apoptosis and enhance the sensitivity of radiotherapy probably via the mechanism of targeting HER-2.

关 键 词：miR-133b基因 HER-2基因 结肠癌细胞系 放射敏感性 

分 类 号：R735.35[医药卫生—肿瘤]