微囊化对重组亚油酸异构酶酶学性质的影响  

EFFECT OF MICROENCAPSULATION ON ENZYMATIC PROPERTIES OF RECOMBINANT LINOLEIC ACID ISOMERASE

作　　者：王丹 智超 柳玫琪 赵微 刘扬[1] 包秋华[2] 张和平[2] 赵国芬[1] WANG Dan;ZHI Chao;LIU Meiqi;ZHAO Wei;LIU Yang;BAO Qiuhua;ZHANG Heping;ZHAO Guofen(College of Life Scieuces,Inner Mongolia Agricultural University,Hohhot 010018,China;College of Food Scieuces&e Engineering,Inner Mongolia Agriculural University,Hohhot 010018,China;School of Chemical&Material Engineering,Wuxi 2141223,China)

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018 [2]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [3]江南大学化学与材料工程学院,无锡2141223

出　　处：《内蒙古农业大学学报（自然科学版）》2021年第2期54-58,共5页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：内蒙古自然科学基金项目(2017MS0306);国家自然科学基金项目“植物乳杆菌P-8亚油酸异构酶结构和功能的研究”(31560443)。

摘　　要：本试验利用层层自组装技术,以聚电解质PAH和PSS为微囊化材料,将以重组菌株JM109-LAI-pQE30表达亚油酸异构酶,纯化后进行微囊化,对微囊化前后的LAI酶学性质研究。试验表明,微囊化LAI时,CaCl2:Na2CO3:LAI的比例为1:1:2时,LAI的固载率达95%以上。微囊化LAI最适反应温度为55℃,最适反应pH为5.5。微囊化的LAI在热稳定性和酸碱稳定性方面优于游离LAI。此研究结果为LAI的进一步应用奠定基础。By using the layer-by-layer self-assembly technology,the recombinant linoleic acid isomerase was expressed with M109-LAI-pQE30 in the experiment.Then,the microencapsulation was conducted with the polyelectrolyte PAH and PSS after purification.At last,the enzymatic properties on LAI was investigated before and after microencapsulation.The results showed that the loading rate of LAI could reach more than 95%when the ratio of CaCl2,Na2CO3 and LAI was 1:1:2.The optimum reaction temperature for microencapsulating LAI was 55℃,and the optimum reaction pH value was 5.5.The microencapsulated LAI was superior to free LAI in the thermal stability and acid-base stability.The results laid the foundation for the further application of LAI.

关 键 词：层层自组装 微囊化 重组亚油酸异构酶 

分 类 号：Q814[生物学—生物工程]