电感耦合等离子体质谱法测定食品中硒元素的条件优化  

Optimization of determination conditions of selenium in food by inductively coupled plasma mass spectrometry

作　　者：赵晨曦[1] 高佳 付志斌[1] 常凤启[1] ZHAO Chen-Xi;GAO Jia;FU Zhi-Bin;CHANG Feng-Qi(Hebei Provincial Center for Disease Control and Prevention,Shijiazhuang 050021,China)

机构地区：[1]河北省疾病预防控制中心,石家庄050021

出　　处：《食品安全质量检测学报》2021年第5期1682-1687,共6页Journal of Food Safety and Quality

基　　金：河北省卫健委跟踪项目(GL2018085)

摘　　要：目的优化电感耦合等离子体质谱法(inductively coupled plasma mass spectrometer, ICP-MS)测定食品中硒元素含量的条件,并对河北省食品中硒元素含量情况进行调查分析。方法选取8种不同基质的国家标准物质作为检测样品对实验条件进行优化,同时选择80Se作为检测目标,采用碰撞模式并加入内标和5%异丙醇的优化条件下对样品中硒元素含量进行检测。通过对比结果,分析河北省膳食样品中硒元素含量,对居民通过膳食补充硒元素给予指导。结果通过采用碰撞模式及内标添加法可以有效降低基质干扰,同时加入异丙醇可极大提高硒元素的信号强度;其在5.00~100.00μg/L范围内呈现良好的线性关系(r2=0.999),检出限为0.002mg/kg;坚果及籽类、豆类及豆制品和肉及肉制品含硒较高,坚果及籽类样品中鲍鱼果含量最高为1.472mg/kg,豆类及豆制品中黄豆含量最高为0.123mg/kg,肉及肉制品中鸭肉含量最高为0.169 mg/kg。结论在检测的6类食品中,坚果及籽类、豆类及豆制品和肉及肉制品中硒含量相对较高,缺硒人群可适量增加食用。Objective To optimize the conditions for the determination of selenium in foods by inductively coupled plasma mass spectrometry(ICP-MS),and to investigate and analyze the selenium content in foods in Hebei Province.Methods Eight national reference materials with different matrices were selected as the test samples,and the experimental conditions were optimized.At the same time,80Se was selected as the test target.The selenium content in the samples was determined under the optimized conditions of collision mode,internal standard and 5%isopropanol.By comparing the results,the content of selenium in dietary samples of Hebei province was analyzed to provide guidance for residents to supplement selenium through diet.Results The matrix interference could be effectively reduced by using collision mode and internal standard addition method,and the signal intensity of selenium could be greatly improved by adding isopropanol;the linear range was 5.00-100.00μg/L(r 2=0.999),and the limit of detection was 0.002 mg/kg.The highest content of selenium in nuts and seeds was 1.472 mg/kg,the highest content of soybean was 0.123 mg/kg,and the highest content of duck meat was 0.169 mg/kg.Conclusion Among the 6 kinds of food tested,the selenium content in nuts and seeds,beans and bean products,meat and meat products is relatively high,and the selenium deficient people can increase their consumption appropriately.

关 键 词：硒 电感耦合等离子体质谱法 优化 

分 类 号：O657.63[理学—分析化学] TS207.3[理学—化学]