表面增强拉曼光谱法快速检测减肥类保健食品中的比沙可啶  

Rapid determination of bisacodyl in weight-loss functional foods by surface enhanced Raman spectroscopy

作　　者：张烨 杨国伟 陈煜 阎安婷 ZHANG Ye;YANG Guo-Wei;CHEN Yu;YAN An-Ting(Shanxi Institute of Food and Drug Control,Taiyuan 030031,China)

机构地区：[1]山西省食品药品检验所,太原030031

出　　处：《食品安全质量检测学报》2021年第5期1778-1782,共5页Journal of Food Safety and Quality

基　　金：山西省重点研发计划项目(201903D311002)。

摘　　要：目的建立表面增强拉曼光谱法(surface enhanced Raman spectroscopy, SERS)快速测定减肥类保健食品中比沙可啶的分析方法。方法激发波长785nm±0.5nm,光谱覆盖范围为300~2300cm~(-1),光谱分辨率为4~10 cm-1,拉曼光谱仪进行测定,最后采用高效液相色谱法(high performance liquid chromatography, HPLC)进行验证。结果该方法的线性范围为0.5~5 mg/L,检出限为0.2 mg/kg,回收率为77.3%~107.2%,相对标准偏差(relative standard deviation,RSD)(n=6)不大于6.3%,检测结果与高效液相色谱法检测的结果无明显差异。结论该方法快速、简便,可作为检测减肥类保健食品中非法添加的比沙可啶的快检方法。Objective To establish a rapid method for the determination of bisacodyl in weight-loss functional foods by surface enhanced Raman spectroscopy(SERS).Methods The excitation wavelength of the experiment was 785 nm±0.5 nm,the spectral coverage was 300-2300 cm‒1,and the spectral resolution was 4~10 cm‒1.Raman spectroscopy was used to determine the content,and high performance liquid chromatography(HPLC)was used for verification finally.Results The linear range of this method was from 0.5 to 5 mg/L,and the limit of detection was 0.2 mg/kg.The good recoveries were in range of 77.3%‒107.2%with relative standard deviations(RSD,n=6)no more than 6.3%.The results showed no significant difference with those of HPLC.Conclusion The method is rapid and simple.It can be used for the detection of bisacodyl in weight-loss functional foods rapidly.

关 键 词：表面增强拉曼光谱法 减肥类保健食品 比沙可啶 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.37[轻工技术与工程—食品科学与工程]