MicroRNA-216b靶向DCLK1调控卵巢癌HO-8910细胞的增殖迁移和侵袭  

MicroRNA-216b Targets DCLK1 Regulating the Proliferation,Migration and Invasion of Ovarian Cancer HO-8910 Cells

作　　者：樊涛[1] 李江鹏[1] FAN Tao;LI Jiangpeng(Xi'an People's Hospital / Xi'an Fourth Hospital, Shanxi Xi'an 710004, China)

机构地区：[1]陕西省西安市人民医院/陕西省西安市第四医院,陕西西安710004

出　　处：《河北医学》2021年第4期534-538,共5页Hebei Medicine

基　　金：陕西省医学科学研究重点课题计划,(编号:2016JM1214)。

摘　　要：目的:探讨MicroRNA-216b(miR-216b)对卵巢癌HO-8910细胞增殖、迁移和侵袭的影响。方法:收集30例卵巢癌患者癌组织和癌旁组织,采用qPCR检测癌组织和癌旁组织miR-216b的表达。采用HO-8910细胞为对照组,转染空载质粒HO-8910细胞为阴性对照组,转染miR-216b mimic的HO-8910细胞为过表达组,转染miR-216b inhibitor HO-8910细胞为低表达组。采用CCK8法、划痕实验、平板克隆、Transwell检测各组细胞增殖、迁移和侵袭能力,采用Western blot和qPCR检测各组细胞双皮质素样激酶1(Doublecortinlike kinase 1,DCLK1)蛋白和mRNA的表达情况。结果:卵巢癌患者癌组织中miR-216b的表达量明显低于癌旁组织(P<0.05);CCK8法、划痕实验、Transwell结果显示,相对于对照组和阴性对照组,miR-216b mimic组细胞增殖、迁移和侵袭能力显著降低(P<0.05),miR-216b inhibitor组显著增加(P<0.05);Western和qPCR结果显示,与对照组和阴性对照组,miR-216b mimic组细胞DCLK1蛋白和mRNA表达水平显著降低(P<0.05),miR-216b inhibitor组显著增加(P<0.05)。结论:miR-216b具有靶向DCLK1,进而抑制卵巢癌HO-8910细胞增殖、迁移和侵袭的作用。miR-216b可能卵巢癌诊断治疗的新靶点。Objective:To investigate the effect of MicroRNA-216b(miR-216b)on the proliferation,migration and invasion of ovarian cancer HO-8910 cells.Methods:The expression of miR-216b in cancer and paracancerous tissues of 30 patients with ovarian cancer was detected by qPCR.Experimental cells were divided into four groups:control group(normal HO-8910 cells),negative control group(transfected empty plasmid HO-8910 cells),overexpression group(transfected miR-216bmimicHO-8910 cells),low expression group(transfected miR-216binhibitorHO-8910 cells).CCK8 assay,scratch test,plate cloning and Transwell were used to detect the ability of cell proliferation,migration and invasion.Westernblot and qPCR were used to detect the expression of biscorticoid kinase 1(Doublecortinlikekinase1,DCLK1)protein and mRNA.Results:The expression of miR-216b in ovarian cancer tissues was significantly lower than that in paracancerous tissues,and the results of CCK8 assay,scratch test and Transwell showed that the ability of cell proliferation,migration and invasion in miR-216bmimic group was significantly lower than that in control group and negative control group,while that in miR-216binhibitor group was significantly higher than that in control group and negative control group.The results of Western and qPCR showed that the expression levels of DCLK1 protein and mRNA in miR-216bmimic group were significantly lower than those in control group and negative control group,while those in miR-216binhibitor group were significantly higher than those in control group and negative control group.Conclusion:MiR-216b can target DCLK1 to inhibit the proliferation,migration and invasion of ovarian cancer HO-8910 cells.MiR-216b may be a new target for diagnosis and treatment of ovarian cancer.

关 键 词：miR-216b 双皮质素样激酶1 卵巢癌HO-8910细胞 增殖 

分 类 号：R73[医药卫生—肿瘤]