新疆维吾尔族溃疡性结肠炎患者相关的长链非编码RNA差异表达谱及功能研究  

Study on the differential expression profile and function of UC-related LncRNA in Uygur population of Xinjiang

作　　者：苏莎莎[1] 孔文洁[2] 莎比亚·沙吾提[1] 张静[1] 郭红梅[1] SU Sha-sha;KONG Wen-jie;SHABIYA Sha-wuti(Department of Gastroenterology,The Fourth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China;不详)

机构地区：[1]新疆医科大学第四附属医院消化科,新疆乌鲁木齐830000 [2]新疆维吾尔自治区人民医院,新疆乌鲁木齐830000

出　　处：《中国医学装备》2021年第4期30-35,共6页China Medical Equipment

基　　金：新疆维吾尔自治区自然科学基金(2017D01C111)“新疆维吾尔族溃疡性结肠炎相关的Lnc RNA筛选及功能研究”。

摘　　要：目的:利用微阵列芯片和生物信息学技术,分析溃疡性结肠炎(UC)中长链非编码RNA(lncRNA)表达特征。方法:选取医院确诊的3例维吾尔族UC患者,将其纳入观察组,另选3名维吾尔族健康体检者纳入对照组,运用lncRNA芯片检测两组结肠组织中lncRNAs和信使核糖核酸(mRNAs)表达谱,分析两组间差异表达的lncRNAs。运用在线生物信息学软件对差异lncRNAs的生物学功能和lncRNAs预测的靶向mRNAs进行全基因组关联分析。结果:芯片结果预处理分析中,两组的芯片结果在总体基因的表达一致。通过对lncRNA和mRNA的数据进行聚类分析发现,在两组样本中,lncRNA和mRNA的基因表达存在明显的差异。观察组患者病变结肠组织中表达的lnc RNAs筛选差异倍数≥2,共有1242个,其中表达上调579个,下调663个;差异表达的lncRNAs主要在免疫系统进展、炎症通路及B细胞的激活等功能上显著富集;基因座信息预测差异表达的lncRNA靶基因中有8个与UC发病机制相关的lncRNA-mRNA分子调控机制。结论:新疆维吾尔族UC患者与维吾尔族健康者的结肠组织存在差异表达的lncRNAs,且可能参与UC的致病调控过程。Objective:To analyze the expression characteristics of long non-coding RNA(lncRNA)in ulcerative colitis(UC)by using microarray chips and bioinformatics technique.Methods:Three Uyghur patients with UC who were diagnosed in the hospital were selected and were divided into the observation group,and another three Uyghur health subjects who were confirmed by physical examination were divided into the control group.The lncRNA chip was used to detect the expression profile of lncRNAs and mRNAs in the colon tissues of two groups,and to analyze the lncRNA of differential expression of two groups.The online bioinformatics software was used to perform genome-wide association analysis on the biological functions of differential lncRNAs and targeted mRNAs predicted by lncRNAs.Results:In the preprocessing analysis of the chip results of the two groups,the overall gene expressions of the chip results of two groups were consistent.The results of cluster analysis of lncRNA and mRNA data indicated that there are significant differences in the gene expression of lncRNA and mRNA between the two groups.The multiple of screening difference of expressed lnc RNAs in the diseased colon tissue of the observation group was≥2,and there were 1242 in total,of which 579 were up-regulated and 663 were down-regulated.Differentially expressed lncRNAs were mainly enriched in series functions included immune system progression,inflammation pathways and B cell activation.In the differentially expressed lnc RNA target genes that were predicted by locus information,8 lncRNA-mRNA molecules regulation mechanism related to the UC pathogenesis.Conclusion:There are differentially expressed lncRNAs in the colon tissues between Xinjiang Uyghur UC patients and Uyghur healthy individuals,and they may be involved in the progress of regulation and control of pathogenesis of UC.

关 键 词：溃疡性结肠炎(UC) 维吾尔族 长链非编码RNA(lncRNA) 差异表达谱 

分 类 号：R574.62[医药卫生—消化系统]