凝结芽孢杆菌的产孢条件及高密度培养工艺  

Optimization of the spore-forming and high density culture conditions for Bacillus coagulans

作　　者：孙标 陈维菁 赵迎庆 卢建军[3] 唐寅芳 张富明 SUN Biao;CHEN Weijing;ZHAO Yingqing;LU Jianjun;TANG Yinfang;ZHANG Fuming(Zhejiang Qirun Biotechnology Limited Company,Jiaxing,Zhejiang 314022,China;Applied Technology Research Institute of Zhejiang Academy of Sciences,Jiaxing,Zhejiang 314022,China;College of Animal Science,Zhejiang University,Hangzhou,Zhejiang 310058,China)

机构地区：[1]浙江启润生物科技有限公司,浙江嘉兴314022 [2]浙江中科院应用技术研究院,浙江嘉兴314022 [3]浙江大学动物科学学院,浙江杭州310058

出　　处：《湖南农业大学学报（自然科学版）》2021年第2期171-179,共9页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：浙江省重点研发项目(2019C02051);浙江中科院应用技术研究院项目(ZK-PY-2019-02)。

摘　　要：为提高凝结芽孢杆菌发酵产孢率,通过单因素试验、Plackett-Burman设计、爬坡试验、中心复合试验对凝结芽孢杆菌高密度发酵工艺进行优化。结果表明:凝结芽孢杆菌最佳培养基组分为麸皮36.91 g/L、玉米浆干粉13.70 g/L、MnSO40.84 g/L,在此条件下凝结芽孢杆菌发酵产芽孢数达到2.848×10^(10)cfu/mL,相比优化前芽孢数提高了61.72%;在优化的培养基基础上进行500 L发酵试验,培养条件为发酵温度40℃、转速150 r/min、通气量2.5 m^(3)/h、接种量5%、pH 7.0、培养时间36 h,发酵液芽孢数达到2.98×10^(10)cfu/mL,与优化试验结果相近,为进一步优化生产工艺提供了必要的数据支撑。In order to improve sporulation rate of Bacillus coagulans,in this study,we investigate the high-density fermentation culture conditions for Bacillus coagulans using a batch experiments including single-factor experiment,Plackett-Burman design experiment,steepest ascent search experiment,response surface experiments with the Box-Behnken design.The results showed that Bran 36.91 g/L,corn steep powder 13.70 g/L,MnSO40.84 g/L were the optimal medium for Bacillus coagulans.Under this condition,the spore number of QR-N323 was 2.848×10^(10)cfu/mL.The number of spores increased by 61.72%compared that before the optimization.The optimized medium components were used for 500 L fermentation scaling-up test with temperature 40℃,Rotating speed 150 r/min,ventilatory capacity 2.5 m^(3)/h,inoculation quantity 5%,pH 7.0 and incubation time 36 h.The results were comparable to the optimized experiment results,which provides necessary data supportting further production process optimization.

关 键 词：凝结芽孢杆菌 高密度发酵 芽孢数 响应面试验 培养基优化 

分 类 号：S939.97[农业科学—渔业资源]