miR-1260a调控FEZ1对前列腺癌细胞增殖、凋亡、迁移和侵袭的影响  

Effects of FEZ1 regulated by miR1260a on proliferation,apoptosis,migration and invasion of prostate cancer cells

作　　者：孙彦申 张跃 SUN Yanshen;ZHANG Yue(Department of Urinary Surgery,Zaozhuang Hospital of Zaozhuang Mining Industry Group,Shandong,Zaozhuang 277100,China)

机构地区：[1]枣庄矿业集团枣庄医院泌尿外科,山东省枣庄市277100

出　　处：《河北医药》2021年第7期970-975,共6页Hebei Medical Journal

摘　　要：目的探究微小RNA-1260a(miR-1260a)调控亮氨酸拉链蛋白(FEZ1)对前列腺癌细胞增殖、凋亡、迁移、侵袭的影响及其作用机制。方法采用实时荧光定量聚合酶链反应(qRT-PCR)检测前列腺癌组织及癌旁组织中miR-1260a的表达。体外培养前列腺癌DU145细胞,实验分成4组:anti-miR-NC组、anti-miR-1260a组、anti-miR-1260a+si-NC组、anti-miR-1260a+si-FEZ1组。甲基噻唑基四唑(MTT)检测4组DU145细胞活力;流式细胞术检测4组DU145细胞凋亡率;Transwell实验检测4组DU145细胞迁移及侵袭能力;双荧光素酶报告系统确认miR-1260a直接调控的靶基因。蛋白免疫印迹(Western blot)检测细胞周期蛋白1(CyclinD1)、B淋巴细胞瘤-2(Bcl-2)、基质金属蛋白酶-2(MMP-2)、P21、B淋巴细胞瘤-2相关蛋白(Bax)、上皮钙黏附素(E-cadherin)、FEZ1蛋白表达。结果与癌旁组织比较,前列腺癌组织中miR-1260a的表达水平显著升高(P<0.01);与anti-miR-NC组比较,anti-miR-1260a组DU145细胞活力显著降低(P<0.01),细胞凋亡率显著升高(P<0.01),Cyclin D1、Bcl-2蛋白表达水平显著降低(P<0.01),P21、Bax蛋白表达量显著升高(P<0.01),迁移与侵袭细胞数显著减少(P<0.01),MMP-2蛋白表表达量显著降低(P<0.01),E-cadherin蛋白表达量显著升高(P<0.01);FEZ1是miR-1260a的靶基因;抑制FEZ1的表达可逆转抑制miR-1260a的表达对DU145细胞增殖、凋亡、迁移、侵袭的影响。结论miR-1260a靶向抑制FEZ1的表达调控前列腺癌细胞的增殖、凋亡、迁移、侵袭。Objective To investigate the effects of microRNA-1260a(miR-1260a)regulation of leucine zipper protein(FEZ1)on proliferation,apoptosis,migration and invasion of prostate cancer cells and its mechanism,and to explore its action mechaniam.Methods Real-time fluorescent quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of miR-1260a in prostate cancer tissues and adjacent normal tissues.Prostate cancer DU145 cells were cultured in vitro,and they were divided into 4 groups:anti-miR-NC group,anti-miR-1260a group,anti-miR-1260a+si-NC group,and anti-miR-1260a+si-FEZ1 group.Methylthiazolyltetrazole(MTT)was used to detect the viability of DU145 cellsin the four groups,and flow cytometry was used to detect the apoptosis rate of DU145 cells in the four groups.The Transwell experiment was used to detect the migration and invasion ability of DU145 cells in the four groups,and the dual luciferase reporter system was used to confirm target genes directly regulated by miR-1260a.In addition,Western blot was used to detect the expressions of Cyclin 1(CyclinD1),B lymphocyte tumor 2(Bcl-2),matrix metalloproteinase 2(MMP-2),P21,B lymphoma 2 related proteins(Bax),epithelial cadherin(E-cadherin),and FEZ1 protein.Results As compared with those in adjacent normal tissues,the expression levels of miR-1260a in prostate cancer tissue were significantly increased(P<0.01).As compared with that in anti-miR-NC group,the viability of DU145 cells in the anti-miR-1260a group was significantly decreased(P<0.01),and the apoptosis rate was significantly increased(P<0.01),and the expression levels of Cyclin D1 and Bcl-2 protein were significantly decreased(P<0.01),and the expression levels of P21 and Bax proteins were significantly increased(P<0.01).In addition,the number of migrating and invasive cells was significantly decreased(P<0.01),the expression levels of MMP-2 protein were significantly decreased(P<0.01),and the expression levels of E-cadherin protein were significantly increased(P<0.01).FEZ1 was the target gene of

关 键 词：前列腺癌 miR-1260a FEZ1 增殖 迁移 侵袭 

分 类 号：R737.25[医药卫生—肿瘤]