组蛋白去甲基化酶含十字形结构域蛋白-3和核因子-κB高表达与肝细胞癌不良进展和预后的关系  

Increased expression of jumonji domain-containing3,and nuclear factor-κB predicts poor progression and prognosis of patients with hepatocellular carcinoma

作　　者：王冰[1] 李锡清[1] 杨建旭[1] 牛标 王维伟[1] 孙建军[1] 魏思东[1] 陈国勇[1] Wang Bing;Li Xiqing;Yang Jianxu;Niu Biao;Wang Weiwei;Sun Jianjun;Wei Sidong;Chen Guoyong(Department of Hepatobiliary Pancreatic Surgery,Henan Provincical People′s Hospital,Zhengzhou 450003,China)

机构地区：[1]河南省人民医院肝胆外科,郑州450003

出　　处：《中华实验外科杂志》2021年第3期542-546,共5页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(U2004124)。

摘　　要：目的观察肝细胞癌中组蛋白去甲基化酶含十字形结构域蛋白-3(JMJD3)和核因子-κB(NF-κB)蛋白水平的表达,探讨两者的相关性及其蛋白表达水平与多个临床病理因素的关系。方法采用实时荧光定量反转录-聚合酶链反应(RT-qPCR)法检测25例新鲜肝细胞癌组织及相对应的癌旁组织中JMJD3和NF-κB信使RNA(mRNA)表达,采用免疫组织化学法检测102例肝细胞癌及相对应的癌旁组织中JMJD3和NF-κB蛋白的表达,分析其与肝细胞癌临床病理特征的关系及上述蛋白的相关性,采用生存分析法(Kaplan-Meier)法来评估JMJD3和NF-κB蛋白的异常表达与肝细胞癌患者生存期之间的关系,Cox回归模型分析JMJD3和NF-κB蛋白的表达和临床病理参数与肝细胞癌预后的关系。结果JMJD3和NF-κB mRNA在肝细胞癌组织中的表达水平均高于癌旁组织(t=-15.130、-15.030,P<0.05),差异有统计学意义,肝细胞癌组织中JMJD3阳性率[87.25%(89/102)]显著高于相应癌旁组织[57.84%(59/102),χ^(2)=22.153,P<0.05],差异有统计学意义,肝细胞癌组织中NF-κB阳性率[83.33%(85/102)]显著高于相应癌旁组织[29.41%(30/102),χ^(2)=55.664,P<0.05],差异有统计学意义。JMJD3和NF-κB表达与甲胎蛋白水平、分化程度、有无脉管癌栓、淋巴结转移及TNM分期明显相关(P<0.05),NF-κB与结节数目明显相关(χ^(2)=5.133,P<0.05),差异有统计学意义。JMJD3蛋白高表达者的生存期高于低表达者(χ^(2)=40.568,P<0.05),差异有统计学意义,NF-κB蛋白高表达者的生存期低于低表达者(χ^(2)=48.803,P<0.05),差异有统计学意义。Spearman相关分析结果显示JMJD3与NF-κB呈正相关(r=0.627,P<0.05)。结论JMJD3和NF-κB在肝细胞癌中的异常表达与肿瘤的浸润、转移及无病生存期明显相关。Objective To observe the expression of jumonji domain-containing3(JMJD3)and nuclear factor-κB(NF-κB)mRNA and protein in hepatocellular carcinoma(HCC),and to explore the correlation between the expression of JMJD3 and NF-κB protein and clinicopathological factors.Methods JMJD3 and NF-κB mRNA and protein levels in 25 fresh hepatocellular carcinoma and its adjacent tissues were detected by quantitative real-time reverse transcription-polymerase chain reaction(RT-qPCR)and immunohistochemistry respectively.The relationship between the expression of JMJD3 and NF-κB proteins and the clinicopathological features of HCC was analyzed.The Kaplan-Meier method was used to evaluate the relationship between the abnormal expression of JMJD3 andNF-κB proteins and the survival time of HCC patients.The Cox-regression model was used to evaluate the prognositic factors,including the expression of JMJD3 and NF-κB proteins and clinicopathological parameters.Results The expression levels of JMJD3 and NF-κB mRNA in HCC tissues were higher than those in adjacent tissues(P<0.05).The positive rate of JMJD3 in HCC tissues[87.25%(89/102)]was significantly higher than that in corresponding adjacent tissues[57.84%(59/102),χ^(2)=22.153,P<0.05].The positive rate of NF-κB in HCC tissues[83.33%(85/102)]was significantly higher than that in corresponding adjacent tissues[29.41%(30/102),χ^(2)=55.664,P<0.05].The expression of JMJD3 and NF-κB was correlated with AFP level,differentiation degree,vascular tumor thrombus,lymph node metastasis and TNM stage(P<0.05),and NF-κB was correlated with lymph node metastasis(χ^(2)=5.133,P<0.05).The survival time of patients with high expression of JMJD3 protein was shorter than that of patients with low expression(χ^(2)=40.568,P<0.05),and that of patients with high expression of NF-κB protein was shorter than that of patients with low expression(χ^(2)=48.803,P<0.05).Spearman correlation analysis showed that JMJD3 was positively correlated with NF-κB(r=0.627,P<0.05).Conclusion The abnormal expressi

关 键 词：肝细胞癌 核因子-ΚB 

分 类 号：R735.7[医药卫生—肿瘤]