石墨烯量子点荧光“开启”适配体传感器检测卡那霉素  

Fluorescent ‘Turn-on’ Aptasensor Based on Graphene Quantum Dot for Detection of Kanamycin

作　　者：张磊[1,2] 邓健康[3] 张富源 ZHANG Lei;DENG Jian-kang;ZHANG Fu-yuan(State Key Laboratory of Food Nutrition and Safety,College of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457,China;School of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China;College of Life Science,Hengshui University,Hengshui 053000,Hebei,China;College of Food Science&Technology,Agriculture University of Hebei,Baoding 071001,Hebei,China)

机构地区：[1]天津科技大学食品科学与工程学院食品营养与安全国家重点实验室,天津300457 [2]河南科技学院食品学院,河南新乡453003 [3]衡水学院生命科学学院,河北衡水053000 [4]河北农业大学食品科技学院,河北保定071001

出　　处：《食品研究与开发》2021年第8期132-139,共8页Food Research and Development

摘　　要：该文开发一种基于结构转换适配体(aptamers)的新型高灵敏度荧光"开启"适配体传感器,用于快速、灵敏检测动物源性食品中卡那霉素(kanamycin,KAN)。适配体的结构转换诱导氮掺杂石墨烯量子点(nitrogen-doped graphene quantum dots,N-GQDs)的聚集/解聚行为,从而引起体系荧光的淬灭/恢复。与之前文献方法相比,该研究方法在效率、灵敏度、选择性和稳定性等方面均表现出优异性能:线性范围为0.1 ng/mL~10.0 ng/mL,检测限低至0.036 ng/mL(S/N=3),远远低于动物源性食品中KAN的最大残留限量。并且整个检测过程(包括样品提取)可在45 min内完成。此外,该方法成功用于5种动物源性食品样品(牛奶、蜂蜜、鱼、蛋、鸡肉)中KAN的检测。Herein,a novel fluorescent‘turn-on’aptasensor based on a structure-switching aptamer was developed for rapid and sensitive detection of kanamycin(KAN)in animal-derived food. Its structure-switching could induce the aggregation/disaggregation behavior of nitrogen-doped graphene quantum dots(N-GQDs),which causes fluorescence quenching/recovery in the system. Compared with previous methods,this novel strategy exhibited superior behavior in terms of efficiency,sensitivity,selectivity,and stability in KAN determination:a linear range from 0.1 ng/mL to 10.0 ng/mL and a satisfactory detection limit as low as 0.036 ng/mL(S/N=3). In addition,the entire method,including sample extraction,could be completed within 45 min. Furthermore,the proposed method was applied to five animal-derived food samples(milk,honey,fish,eggs,and chicken muscle),which demonstrated practical applicability.

关 键 词：石墨烯量子点 互补链 荧光适配体传感器 卡那霉素 结构转换 

分 类 号：O657.3[理学—分析化学] TS207.5[理学—化学] TP212[轻工技术与工程—食品科学]