慢性前列腺炎患者尿道分泌物微生物组学分析  

Microorganisms in the urethral secretions of chronic prostatitis patients:A genomic analysis

作　　者：吴意 谭明波 姜海洋[2] 陆学东[2] 李显文 杨逢生 WU Yi;TAN Ming-bo;JIANG Hai-yang;LU Xue-dong;LI Xian-wen;YANG Feng-sheng(Department of Clinical Laboratory,Shenzhen Yantian People's Hospital,Shenzhen,Guangdong 518081,China;Department of Urology,The Eighth Affiliated Hospital of Sun Yat-sen University,Shenzhen,Guangdong 518033,China)

机构地区：[1]深圳市盐田区人民医院检验科,广东深圳518081 [2]中山大学附属第八医院泌尿外科,广东深圳518033

出　　处：《中华男科学杂志》2021年第2期114-123,共10页National Journal of Andrology

基　　金：深圳市盐田区科技局科技计划资助项目(20160202)。

摘　　要：目的:探讨慢性前列腺炎(CP)患者尿道分泌物中微生物菌群结构差异,为CP的诊治提供可靠的病原学依据。方法:运用高通量二代测序技术检测33例CP患者尿道分泌物中的微生物,同时以30例健康男性作为对照组;采用秩和检验进行组间显著性差异分析,使用生物信息学软件进行数据处理,以P<0.05为差异有统计学意义。结果:在种分类水平CP组尿道分泌物与健康对照组有17种菌存在显著性差异;LEFSe分析显示CP患者尿道分泌物中丰度差异显著的微生物是微球菌科、红蝽菌科、红蝽菌目、Coriobacteriia、Weeksellaceae、丛毛单胞菌科、肠杆菌科、肠杆菌目、黄单胞菌科、黄单胞菌目和银耳纲;PCA分析显示两组菌群结构差异显著。结论:CP与尿道菌群结构改变存在一定的相关性;CP可能不是由单一的微生物引起的,而是多种微生物共同作用的结果。Objective:To investigate the differences in the microbial floras in the urethral secretions of the patients with chronic prostatitis to provide some reliable pathogenic evidence for the diagnosis and treatment of the disease.Methods:Using highthroughput second-generation sequencing technology,we detected the microorganisms in the urethral secretions from 33 chronic prostatitis patients and 30 normal healthy males.We analyzed the significant differences in the microbial flora between the two groups via the rank-sum test and performed data processing with the bioinformatics software,P<0.05 considered as with statistically significant difference.Results:Statistically significant differences were observed in 17 kinds of bacteria from the urethral secretions between the normal healthy controls and chronic prostatitis patients.LEfSe analysis showed that the microorganisms with most significant abundance difference in the urethral secretions of the chronic prostatitis patients included micrococcaceae,coriobacteriaceae,coriobacteriales,coriobacteriia,weeksellaceae,comamonadaceae,enterobacteriaceae,enterobacteriales,xanthomonadaceae,and xanthomonadales.Principal component analysis(PCA)revealed significant difference in the microbial composition between the two groups.Conclusion:There is a certain correlation between chronic prostatitis and changes in the composition of urethral microbial floras.Chronic prostatitis may result from concerted action of multiple microbes rather than a single one.

关 键 词：慢性前列腺炎 高通量 二代测序 尿道分泌物 微生物学 组学 

分 类 号：R372[医药卫生—病原生物学]