G1抑制胶质瘤细胞增殖的初步研究  

Inhibitory Effect of G1 on the Proliferation in Glioma Cells

作　　者：贺旭[1] 熊英[2] HE Xu;XIONG Ying(Experimental Center for Basic Medical Teaching,Capital Medical University,Beijing,100069,China;Department of Biochemistry and Molecular Biology,Capital Medical University,Beijing,100069,China)

机构地区：[1]首都医科大学基础医学院细胞与分子生物学实验教学中心,北京市100069 [2]首都医科大学基础医学院生物化学与分子生物学系,北京市100069

出　　处：《医学分子生物学杂志》2021年第2期110-115,共6页Journal of Medical Molecular Biology

基　　金：国家自然科学基金(No.81972732)。

摘　　要：目的 研究探讨G蛋白偶联雌激素受体(G protein-coupled estrogen receptor,GPER)的特异性激动剂G1对胶质瘤细胞增殖的作用.方法 筛选收集TCGA数据库和GEO数据库中胶质瘤患者的基因表达谱数据,运用SPSS软件对胶质瘤发生率的性别差异进行二项式检验分析;免疫印迹(Western blot,WB)法检测不同胶质瘤细胞中GPER的内源性表达情况;CCK-8法检测G1刺激胶质瘤细胞后对细胞增殖的影响;结果 女性胶质瘤的发生率显著性的低于男性;胶质瘤细胞中有内源性GPER的表达;G1以剂量依赖及时间依赖的方式抑制胶质瘤细胞增殖;G1抑制胶质瘤细胞增殖的效应可以被G15(GPER特异性拮抗剂)显著性抑制(P<0.01).结论 G1可以经GPER介导抑制胶质瘤细胞的增殖.Objective To examine the effect of GPER agonist G1 on the proliferation of glioma cells.Methods The data were collected and selected from the Cancer Genome Altas(TCGA)database and Gene Expression Omnibus(GEO)database,and the gender difference in the incidence of gliomas was analyzed by SPSS.Western blotting was used to detect the protein levels of GPER in glioma cells.CCK-8 assay was employed to detect the proliferation of glioma cells induced by G1,G1/G15.Results Analysis of GEO and TCGA data showed that the incidence of glioma had significant gender differenc.Glioma had endogenous expression of GPER.G1 was found to significantly inhibit the proliferation of glioma cells in a dose-and time-dependent manner(P<0.01).GPER-specific antagonist G-15 blocked this effect.Conclusion G1 inhibits cell proliferation of glioma cells by GPER.

关 键 词：G1 胶质瘤细胞 细胞增殖 GPER 

分 类 号：Q78[生物学—分子生物学]