Cyanidin 3-glucoside modulated cell cycle progression in liver precancerous lesion,in vivo study  

作　　者：Marwa Matboli Amany H Hasanin Reham Hussein Sarah El-Nakeep Eman K Habib Rawan Ellackany Lobna A Saleh 

机构地区：[1]Department of Biochemistry,Ain Shams Faculty of Medicine,Cairo 11318,Egypt [2]Department of Clinical Pharmacology,Ain Shams Faculty of Medicine,Cairo 11381,Egypt [3]Department of General Internal Medicine,Ain Shams Faculty of Medicine,Cairo 11381,Egypt [4]Department of Anatomy&Embryology,Ain Shams Faculty of Medicine,Cairo 11318,Egypt [5]Department of Undergraduate,Faculty of Medicine,Modern University for Technology and Information,Cairo 11381,Egypt

出　　处：《World Journal of Gastroenterology》2021年第14期1435-1450,共16页世界胃肠病学杂志（英文版）

摘　　要：BACKGROUND Cyanidin-3-O-glucoside(cyan)exhibits antioxidant and anticancer properties.The cell cycle proteins and antimitotic drugs might be promising therapeutic targets in hepatocellular carcinoma.AIM To investigate the effect of cyan administration on cell cycle in hepatic precancerous lesion(PCL)induced by diethylnitrosamine/2-acetylaminofluorene(DEN/2-AAF)in Wistar rats.METHODS In vivo,DEN/2-AAF-induced hepatic PCL,rats were treated with three doses of cyan(10,15,and 20 mg/kg/d,for four consecutive days per week for 16 wk).Blood and liver tissue samples were collected for measurement of the followings;alpha fetoprotein(AFP)liver function and RNA panel differential expression was evaluated via real time polymerase chain reaction.Histopathological examination of liver sections stained with H&E and immunohistochemical study using glutathione S-transferase placental(GSTP)and proliferating cell nuclear antigen(PCNA)antibodies were assessed.RESULTS Cyan administration mitigated the effect of DEN/2-AFF induced PCL,decreased AFP levels,and improved liver function.Remarkably,treatment with cyan dose dependently decreased the long non-coding RNA MALAT1 and tubulin gamma 1 mRNA expressions and increased the levels of miR-125b,all of which are involved in cell cycle and mitotic spindle assembly.Of note,cyan decreased GSTP foci percent area and PCNA positively stained nuclei.CONCLUSION Our results indicated that cyan could be used as a potential therapeutic agent to inhibit liver carcinogenesis in rat model via modulation of cell cycle.

关 键 词：Hepatocellular carcinoma therapy Hepatocellular-carcinoma growth Hepatocellular-carcinoma model Hepatocellular-carcinoma size 

分 类 号：R735.7[医药卫生—肿瘤]