饲粮中添加枸杞多糖对断奶大鼠生长性能、抗氧化能力、肠黏膜免疫功能以及肠道形态结构的影响  

Effects of Dietary Lycium barbarum Polysaccharide on Growth Performance,Antioxidant Capacity,Intestinal Mucosal Immune Function and Intestinal Morphology of Weaned Rats

作　　者：尹业鑫 王芳 余东明 王鑫 符晨星[1] 陈家顺[1,2] YIN Yexin;WANG Fang;YU Dongming;WANG Xin;FU Chenxing;CHEN Jiashun(Animal Nutritional Genome and Germplasm Innovation Research Center,College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China;Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China)

机构地区：[1]湖南农业大学动物科学技术学院,动物营养基因组与种质创新研究中心,长沙410128 [2]中国科学院亚热带农业生态研究所,中国科学院亚热带农业生态过程重点实验室,长沙410125

出　　处：《动物营养学报》2021年第4期2302-2310,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金青年科学基金项目(31702126);中国科学院亚热带农业生态过程重点实验室开放基金(ISA2020101);湖南农业大学青年科学基金项目(19QN01)。

摘　　要：本试验旨在研究饲粮中添加枸杞多糖(LBP)对断奶大鼠生长性能、抗氧化能力、肠黏膜免疫功能以及肠道形态结构的影响。选取健康雄性SD大鼠20只,按体重接近的原则随机分成2组(每组10个重复,每个重复1只),分别饲喂基础饲粮(对照组)和在基础饲粮中添加4 g/kg LBP的试验饲粮(LBP组),试验期28 d。结果显示:1)与对照组相比,LBP组平均日增重(ADG)提高了16.3%(P<0.05)。2)与对照组相比,LBP组血清中总抗氧化能力(T⁃AOC)与谷胱甘肽过氧化物酶(GSH⁃Px)活性分别提高了52.8%(P<0.05)和67.5%(P<0.05),丙二醛(MDA)含量降低了31.7%(P<0.05);LBP组空肠黏膜中T⁃AOC提升了99.1%(P<0.05),MDA含量降低了13.7%(P<0.05)。3)与对照组相比,LBP组空肠黏膜中白细胞介素-2(IL⁃2)与白细胞介素-10(IL⁃10)含量分别上升了31.6%(P<0.05)和66.0%(P<0.05)。4)与对照组相比,LBP组空肠黏膜中免疫球蛋白A(IgA)与免疫球蛋白G(IgG)含量分别提高了33.7%(P<0.05)和35.1%(P<0.05)。5)与对照组相比,LBP组空肠的绒毛高度呈上升的趋势,隐窝深度呈下降趋势,绒毛高度/隐窝深度(V/C)显著增加(P<0.05)。由此可见,饲粮中添加LBP可以提高断奶大鼠的抗氧化能力及肠黏膜免疫功能,改善肠道形态结构,进而达到提高生长性能的效果。The objective of the present study was to investigate the effects of dietary Lycium barbarum polysac⁃charide(LBP)on growth performance,antioxidant capacity,intestinal mucosal immune function and intestinal morphology of weaned rats.Twenty healthy weaned male SD rats,based on the similar body weight,were ran⁃domly assigned to two groups with ten replicates of one rat each.The rats in control groups were fed a basal di⁃et,and those in LBP group were fed an experiment diet which supplemented with 4 g/kg LBP based on the basal diet.The experiment lasted for 28 d.The results showed as follows:1)compared with the control group,the average daily gain(ADG)in the LBP group was increased by 16.3%(P<0.05).2)Compared with the control group,the serum total antioxidant capacity(T⁃AOC)and glutathione peroxidase(GSH⁃Px)activity in the LBP group were increased by 52.8%(P<0.05)and 67.5%(P<0.05),respectively,and the malondialde⁃hyde(MDA)content was reduced by 31.7%(P<0.05);the T⁃AOC in jejunal mucosa in the LBP group was increased by 99.1%(P<0.05),and the MDA content was reduced by 13.7%(P<0.05).3)Compared with the control group,the contents of interleukin⁃2(IL⁃2)and interleukin⁃10(IL⁃10)in jejunal mucosa in the LBP group were increased by 31.6%(P<0.05)and 66.0%(P<0.05),respectively.4)Compared with the control group,the contents of immunoglobulin M and immunoglobulin G in jejunal mucosa in the LBP group were increased by 33.7%(P<0.05)and 35.1%(P<0.05),respectively.5)Compared with the control group,the villi height of jejunum in the LBP group showed an upward trend,the crypt depth showed a down⁃ward trend,and the villi height/crypt depth(V/C)was significantly increased(P<0.05).It is concluded that adding LBP to the diet of weaned rats can improve the antioxidant capacity and immune function of jejunal mu⁃cosa,improve the morphology of jejunum,and them improve the growth performance of weaned rats.

关 键 词：枸杞多糖 断奶大鼠 生长性能 抗氧化能力 免疫功能 肠道形态结构 

分 类 号：S816[农业科学—饲料科学]