菜薹BrWRKY57的特性及其对BrPPH1和BrNCED3的调控  

Characteristics of the Transcription Factor BrWRKY57 and Its Regulation on BrPPH1 and BrNCED3

作　　者：曾泽湘 肖显梅 谭小丽 范中奇 陈建业 ZENG Zexiang;XIAO Xianmei;TAN Xiaoli;FAN Zhongqi;CHEN Jianye(Engineering Research Center of Southern Horticultural Products Preservation,Ministry of Education/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables,College of Horticulture,South China Agricultural University,Guangzhou 510642,China;Key Laboratory of Postharvest Biology for Subtropical Special Agricultural Products of Fujian Province,College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]华南农业大学园艺学院,南方园艺产品保鲜教育部工程研究中心/广东省果蔬保鲜重点试验室,广州510642 [2]福建农林大学食品科学学院,亚热带特色农产品采后生物学福建省高校重点试验室,福州350002

出　　处：《园艺学报》2021年第3期518-530,共13页Acta Horticulturae Sinica

基　　金：国家自然科学基金面上项目(31671897)。

摘　　要：从菜薹叶片中分离获得1个WRKY转录因子,命名为BrWRKY57。氨基酸序列比对及进化树分析发现BrWRKY57与拟南芥AtWRKY57同源性较高,含有1个WRKY保守结构域,且同属于WRKY转录因子家族Ⅱc亚族。实时荧光定量PCR分析表明,BrWRKY57随着菜薹叶片衰老表达水平升高,外源脱落酸(abscisic acid,ABA)处理显著诱导其表达。亚细胞定位和转录活性分析表明,BrWRKY57定位于细胞核,且在酵母和烟草体内都具有转录激活活性。双荧光素酶瞬时表达试验显示,BrWRKY57可以激活叶绿素降解相关基因BrPPH1和ABA合成相关基因BrNCED3的启动子活性。以上研究结果表明,BrWRKY57参与菜薹叶片衰老过程可能与影响叶绿素降解和ABA合成相关基因的表达有关。In this study,a WRKY transcription factor termed BrWRKY57,was obtained from Chinese flowering cabbage. Amino acid sequence alignment and phylogenetic analysis revealed that BrWRKY57 contained one WRKY conserved domain,exhibited the highest homology with Arabidopsis thaliana AtWRKY57,and belonged to sub-group IIc. Real-time quantitative PCR analysis demonstrated that BrWRKY57 was up-regulated during leaf senescence of Chinese flowering cabbage,and its expression was significantly enhanced by abscisic acid(ABA)treatment. Subcellular localization and transcriptional activity analysis suggested that BrWRKY57 was a nuclear protein and had transcriptional activation activity. Moreover,dual-luciferase transient expression assay revealed that BrWRKY57 could activate the promoter activities of chlorophyll catabolic gene BrPPH1 and ABA biosynthetic gene BrNCED3. These results indicate that BrWRKY57 might be associated with Chinese flowering cabbage leaf senescence via effecting the genes expression of chlorophyll degradation and ABA synthesis.

关 键 词：菜薹 叶片衰老 脱落酸 WRKY转录因子 转录调控 

分 类 号：S634.5[农业科学—蔬菜学]