磷酸肌酸钠对高糖培养的心肌细胞凋亡与IL-6、TNF-α表达的影响  

Effect of Creatine Phosphate Sodium on Cardiomyocyte Apoptosis and Expression of IL-6 and TNF-αCultured in High Glucose

作　　者：王玉静 弥娜 张文强 谢晓莉 侯云飞 杨巧妮 WANG Yu-jing;MI Na;ZHANG Wen-qiang;XIE Xiao-li;HOU Yun-fei;YANG Qiao-ni(Department of Cardiology,the First Affiliated Hospital of Air Force Military Medical University,Xi'an,Shaanxi,710016,China;Department of Cardiology,986th Hospital of Air Force,Xi'an,Shaanxi,710000,China)

机构地区：[1]空军军医大学第一附属医院心内科,陕西西安710016 [2]空军第九八六医院心内科,陕西西安710000

出　　处：《现代生物医学进展》2021年第6期1036-1039,共4页Progress in Modern Biomedicine

基　　金：陕西省社会发展领域项目(2020SF-263)。

摘　　要：目的:探讨磷酸肌酸钠对高糖培养的心肌细胞凋亡与白介素(Interleukin,IL)-6、肿瘤坏死因子(Tumor necrosis factor,TNF)-α表达的影响。方法:SD大鼠心肌细胞分为三组-正常组、心衰组、磷酸肌酸钠组,心力衰竭组用含血清的高糖DMEM培养基(33 mmol/L葡萄糖)培养;磷酸肌酸钠组用含血清的高糖DMEM培养基(33 mmol/L葡萄糖)和100μmol/L磷酸肌酸钠培养;正常组用含10%血清的DMEM培养基(5.5 mmol/L葡萄糖)培养。采用MTT法检测细胞增殖指数,流式细胞术检测细胞凋亡指数,酶联免疫检测上清IL-6、TNF-α含量,Western blot法检测半胱氨酸蛋白酶-3(Caspase-3)、B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)蛋白水平。结果:处理后24 h、48 h,磷酸肌酸钠组心肌细胞增殖指数高于心衰组(P<0.05),细胞凋亡指数、Caspase-3和Bcl-2蛋白、IL-6、TNF-α含量相对表达量低于心衰组(P<0.05),与正常组对比差异无统计学意义(P>0.05)。结论:磷酸肌酸钠在大鼠心肌细胞心力衰竭模型中的应用能降低心肌细胞Caspase-3、Bcl-2蛋白表达,抑制细胞凋亡,提高细胞增殖指数。Objective:To investigate the effects of creatine phosphate sodium on cardiomyocyte apoptosis and the expression of interleukin(IL)-6 and tumor necrosis factor(TNF)-αin high glucose cultured.Methods:The cardiomyocytes of SD rats were divided into three groups-normal group,heart failure group,and sodium creatine phosphate group.The heart failure group was cultured with serum-containing high glucose DMEM medium(33 mmol/L glucose);creatine phosphate sodium group was cultured with serum-containing high glucose DMEM medium(33 mmol/L glucose)and 100μmol/L creatine phosphate sodium;the normal group was cultured with 10%serum-containing DMEM medium(5.5 mmol/L glucose).MTT method was used to detect cell proliferation index;flow cytometry was used to detect cell apoptosis index;enzyme-linked immunosorbent assay was used to detect the content of IL-6 and TNF-αin the supernatant;Western blot method was used to detect Caspase-3,B-cell lymphoma-2(Bcl-2)protein level.Results:24 h and 48 h after treatment,the proliferation indexes of myocardial cells in the sodium phosphate creatine group were higher than those in the heart failure group(P<0.05),and the apoptosis index,Caspase-3 and Bcl-2 protein,IL-6,TNF-αThe relative expression ofαcontent were lower than those in the heart failure group(P<0.05),and there was no significant difference compared with the normal group(P>0.05).Conclusions:The application of creatine phosphate sodium in rat cardiomyocyte heart failure model can reduce the expression of Caspase-3 and Bcl-2 protein in cardiomyocytes,inhibit cell apoptosis and increase cell proliferation index.

关 键 词：磷酸肌酸钠 心肌细胞 心衰 白介素-6 肿瘤坏死因子-Α 

分 类 号：R-33[医药卫生] R587.1