应用实时LAMP方法检测荆条蜜源成分  

Application of Real-time LAMP Method to DetectHoney-source Components of Vitex Negundo

作　　者：张永清[1] 周艺佳 李梦媛 宋春美[1] 郭孝辉[1] ZHANG Yongqing;ZHOU Yijia;LI Mengyuan;SONG Chunmei;GUO Xiaohui(Henan Engineering Lab of Products and Equipments for Rapid Detection of Biomarkers,Henan Key Laboratory of Biomarker Based Rapid-detection Technology for Food Safety,Food and Pharmacy College,Xuchang University,Xuchang 461000,China)

机构地区：[1]许昌学院食品与药学院,河南省食品安全生物标识快检技术重点实验室,生物标识快检产品与装备河南省工程实验室,河南许昌461000

出　　处：《许昌学院学报》2021年第2期70-73,共4页Journal of Xuchang University

基　　金：河南省科技计划项目(182102110285,202102310468);河南省高校省级大学生创新创业训练计划项目(S202010480020)。

摘　　要：针对荆条转录间隔区设计一套引物,通过温度优化、特异性和灵敏度试验,建立一种荆条蜜源DNA的实时环介导等温扩增(LAMP)检测方法.结果显示,LAMP检测方法在温度为59℃时,引物能够将荆条、椴树、大枣和洋槐DNA区分开,灵敏度可达100 pg·μL^(-1).LAMP方法能对荆条蜜源成分进行快速有效检测,为荆条蜜真实性检测提供一种快速准确的检测方法.A real-time loop-mediated isothermal amplification(LAMP)method for the detection of DNA from Vitex negundo honey source was established by designing a set of primers for transcription interval.The results showed that the LAMP method could distinguish DNA from brass,linden,jujube and locust at 59℃with a sensitivity of 100 pg·μL^(-1).LAMP method can quickly and effectively detect the source components of Vitex negundo,providing a fast and accurate detection method for the authenticity detection of Vitex negundo.

关 键 词：荆条蜜 基因 环介导等温扩增 掺假 

分 类 号：Q81[生物学—生物工程]