急性髓系白血病患者血清miR-370、miR-203的表达及临床意义  

Expression and Clinical Significance of Serum MiR-370 and MiR-203 in Patients with Acute Myeloid Leukemia

作　　者：叶丽花 马潇 许树才 YE Li-Hua;MA Xiao;XU Shu-Cai(Department of Oncology and Hematology,Hubei Provincial Hospital of Integrated Chinese and Western Medicine,Wuhan 430015,Hubei Province,China)

机构地区：[1]湖北省中西医结合医院肿瘤血液内科,湖北武汉430015

出　　处：《中国实验血液学杂志》2021年第2期445-449,共5页Journal of Experimental Hematology

摘　　要：目的:探讨microRNA-370(miR-370)、microRNA-203(miR-203)在急性髓系白血病(AML)患者血清中的表达情况,并分析其临床诊断和预后意义。方法:选取AML患者57例为实验组,健康体检者21例作为对照组。采集各组人员的空腹静脉血,采用实时荧光定量PCR法检测各组miR-370、miR203的表达量。绘制受试者工作特征(ROC)曲线以评估血清miR-370、miR-203的诊断值,Kaplan-Meier方法用于估计表达与总生存率的关系。结果:与健康对照组比较,AML患者血清miR-370表达水平显著降低(P<0.05),血清miR-203表达亦显著降低(P<0.05)。ROC曲线分析表明,血清miR-370、miR-203的表达均可用于区分AML患者与健康者,miR-370的ROC曲线下面积为0.909,灵敏度和特异性分别为91.46%和100.00%;miR-203的ROC曲线下面积为0.895,灵敏度和特异性分别为83.45%和89.71%。结果显示,血清miR-370、miR-203水平与AML患者的总体存活率密切相关。结论:miR-370、miR-203在AML患者的血清中表达降低,有可能作为AML诊断和预后的新标志物。Objective:To investigate the expression of microRNA-370(miR-370)and microRNA-203(miR-203)in the serum of patients with acute myeloid leukemia(AML),and to analyze its clinical diagnosis and prognostic significance.Methods:57 patients with acute myeloid leukemia were enrolled as experimental group,and 21 healthy people were enrolled as control group.The fasting venous blood of the personal in the two groups were collected.The expression of miR-370 and miR-203 of the personal in each groups were detected by real-time fluorescent quantitative PCR.The receiver operating characteristic(ROC)curve was plotted to detected the diagnostic values of serum miR-370,miR-203,and the Kaplan-Meier method was used to estimate the relationship between expression and overall survival of the patients.Results:Compared with healthy controls,serum miR-370 expression was significantly decreased in AML patients(P<0.05),and serum miR-203 expression was also significantly decreased(P<0.05).ROC curve analysis showed that the expression of serum miR-370 and miR-203 could be used to distinguish acute myeloid leukemia and healthy people.The area under the ROC curve of miR-370 was 0.909,and the sensitivity and specificity were91.46%and 100.00%,respectively.The area under the ROC curve of miR-203 was 0.895,and the sensitivity and specificity were 83.45%and 89.71%,respectively.Serum levels of miR-370 and miR-203 were closely related to overall survival in AML patients.Conclusion:The expression of miR-370 and miR-203 is decreased in the serum of patients with AML and may be a new markers for the diagnosis and prognosis of AML.

关 键 词：急性髓系白血病 miR-370 miR-203 

分 类 号：R733.71[医药卫生—肿瘤]