长链非编码RNA-TUC338通过PI3K/AKT信号通路对淋巴瘤细胞迁移和增殖的影响  

Effects of Long Non-coding RNA-TU C338 on the Migration and Prolife ration of Lymphoma Cells via PI3K/AKT Signaling Pathway

作　　者：贾振薇[1] 李焱[1] 孔晓阳 赵洪波[1] 杨志峰[1] 叶敬伟 崔桂荣[1] 罗建民[2] JIA Zhen-Wei;LI Yan;KONG Xiao-Yang;ZHAO Hong-Bo;YANG Zhi-Feng;YE Jing-Wei;CUI Gui-Rong;LUO Jian-Min(Department of Hematology,Handan First Hospital,Handan 056002,Hebei Province,China;Department of Hematology,The Second Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei Province,China)

机构地区：[1]河北省邯郸市第一医院血液科,河北邯郸056002 [2]河北医科大学第二医院血液科,河北石家庄050000

出　　处：《中国实验血液学杂志》2021年第2期494-499,共6页Journal of Experimental Hematology

摘　　要：目的:探讨长链非编码RNA-TUC338对淋巴瘤细胞增殖和迁移的影响。方法:采用荧光定量PCR检测不同淋巴瘤细胞中TUC338表达,磺酰罗丹明B实验检测细胞增殖能力,细胞迁移侵袭实验检测淋巴瘤细胞迁移能力,蛋白免疫印迹实验检测PI3K/AKT信号通路蛋白表达情况。结果:与人正常T淋巴细胞H9相比,淋巴瘤细胞Daudi、U937、BC-3和Raji中TUC338表达水平均明显提高(t=13.277、10.103、16.200和26.687,P=0.002、0.005、0.001和0.000)。与NC-siRNA组相比,TUC338-siRNA组穿过小室细胞数明显减少(t=30.508,P=0.000),p-PI3K和p-AKT蛋白表达量明显下降(t=16.872、18.371,P=0.000、0.000),在24、48和72 h的OD_(530)吸光值均明显偏低(P<0.05)。结论:在淋巴瘤细胞中TUC338表达量明显增加,沉默TUC338表达能够有效抑制PI3K/AKT信号通路的激活,进而抑制淋巴瘤细胞的增殖和迁移,在淋巴瘤诊断和治疗中具有潜在应用价值。Objective:To investigate the effect of long non-coding RNA-TUC338 on the proliferation and migration of lymphoma cells.Methods:The expression of TUC338 in different lymphoma cells was detected by fluorescence quantitative PCR,cell proliferation by sulforhodamine B(SRB)assay,migration of lymphoma cells by transwell assay,and protein expression in PI3 K/AKT signaling pathway by Western blot.Results:The expression levels of TUC338 in lymphoma cells Daudi,U937,BC-3,and Raji significantly increased in comparison with human normal T lymphocytes H9(t=13.277,10.103,16.200,and 26.687,P=0.002,0.005,0.001,and 0.000).Compared with NC-siRNA group,the number of cells crossing the chamber of TUC338-siRNA group was significantly reduced(t=30.508,P=0.000),the protein expression levels of p-PI3 K and p-A.KT significantly decreased(t=16.872 and 18.371,P=0.000 and 0.000),and OD530 absorbance values at 24 h,48 h,and 72 h were significantly lower(P<0.05).Conclusion:The expression of TUC338 significantly increases in lymphoma cells,and silence of TUC338 effectively inhibits the activation of PI3 K/AKT signaling pathway,thereby inhibiting the proliferation and migration of lymphoma cells,which has a potential application value in diagnosis and treatment of lymphoma.

关 键 词：长链非编码RNA TUC338 淋巴瘤 增殖 迁移 PI3K/AKT信号通路 

分 类 号：R733.1[医药卫生—肿瘤]