自噬对B细胞淋巴瘤细胞耐药性的影响  

Effect of Autophagy to Drug Resistance of B-cell Lymphoma Cells

作　　者：周仕霞 张力 李晓明 唐君玲 ZHOU Shi-Xia;ZHANG Li;LI Xiao-Min;TANG Jun-Ling(Department of Hematology,Affiliated Hospital of Southwest Medical University,Luzhou 646000,China;Stem Cell Laboratory Affiliated to Southwest Medical University,Luzhou 646000,China;Department of Oraland Maxillofacial Surgery,Stomatological Hospital Affiliated to Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学附属医院血液内科,四川泸州646000 [2]西南医科大学附属医院干细胞实验室,四川泸州646000 [3]西南医科大学口腔医学院口腔颌面外科,四川泸州646000

出　　处：《中国实验血液学杂志》2021年第2期500-507,共8页Journal of Experimental Hematology

基　　金：四川省重点研发项目(2019YFS0301);四川省卫生健康委员会重点研究项目(18ZD014);西南医科大学附属医院博士科研启动基金(19032,19079);泸州市科技局应用基础研究项目(2019LZXNYDJ54);北京医学奖励基金会(YJHYXKYJJ-602);泸州市科技厅应用基础研究项目(2020LZXNYDJ48)。

摘　　要：目的:探讨细胞自噬对不同种人源淋巴瘤细胞耐药性的影响。方法:以人Burkitt’s淋巴瘤细胞Daudi、人B淋巴瘤细胞SUDHL-4和人套细胞淋巴瘤细胞Je Ko-1为研究对象,通过自噬抑制剂处理细胞和慢病毒稳定感染干扰Atg5基因的表达,改变B细胞淋巴瘤细胞的自噬活性,并观察淋巴瘤细胞对ADR和VCR的耐药性的变化。结果:Je Ko-1细胞对ADR和VCR具有较强的耐药性,SUDHL-4次之,Daudi细胞对ADR和VCR的耐药性最弱。同时,Je Ko-1细胞自噬活性也最强,SUDHL-4次之,Daudi细胞自噬活性最弱。自噬抑制剂处理或稳定干扰Atg5基因的表达后,淋巴瘤细胞对ADR和VCR的耐药性显著降低,且耐药性降低程度与淋巴瘤细胞本身自噬活性呈正相关。结论:自噬可以增强淋巴瘤细胞Daudi、SUDHL-4和Je Ko-1对ADR和VCR的耐药性,自噬活性越高,淋巴瘤细胞的耐药性越强。Objective:To investigate the effect of autophagy on the drug resistance of different human lymphoma cells.Methods:Human Burkitt’s lymphoma cell Daudi,human B lymphoma cell SUDHL-4,and human mantle cell lymphoma cell JeKo-1 were taken as the research subjects.The expression of Atg5 was inhibited by the treatments of autophagy inhibitors or stable interference via lentivirus infection.The autophagy activity of B lymphoma cell was changed,and the changes of lymphoma cells to the drug resistance of ADR and VCR was observed.Results:JeKo-1 cells showed the strongest resistance to ADR and VCR,followed by SUDHL-4,and Daudi cells showed the weakest resistance to ADR and VCR.At the same time,JeKo-1 cells showed the strongest autophagy activity,followed by SUDHL-4,and Daudi cells showed the weakest autophagy activity.After the treatments of autophagy inhibitors or stable Atg5 interference,the resistance of lymphoma cells to ADR and VCR was significantly weakened,and there was the positive correlation at the drug resistance and the autophagy activity of B lymphoma cell.Conclusion:The higher autophagy activity in lymphoma cells,the lower chemotherapy resistance of the lymphoma cells after autophagy was inhibited.

关 键 词：淋巴瘤细胞 耐药性 自噬 Atg5 

分 类 号：R733.1[医药卫生—肿瘤]