凋亡刺激蛋白抑制因子、逮捕缺陷蛋白表达与鼻咽鳞状细胞癌病理分期及组织分型关系  

Relationship between the expression of ARD1 and iASPP protein and the development of nasopharyngeal squamous cell carcinoma

作　　者：许金中 王海玲[1] 王真[1] 张有利 李颖[2] XU Jinzhong;WANG Hailing;WANG Zhen;ZHANG Youli;LI Yin(Department of Otolaryngology,Second People's Hospital of Jiyuan,Jiyuan,Henan 459000,China;Department of Otolaryngology,Children's Hospital Affiliated to Zhengzhou University,Zheng-zhou,Henan 450007,China)

机构地区：[1]济源市第二人民医院耳鼻喉科,河南济源459000 [2]郑州大学附属儿童医院、河南省儿童医院、郑州儿童医院耳鼻咽喉科,河南郑州450007

出　　处：《安徽医药》2021年第5期959-962,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨鼻咽鳞状细胞癌组织中的凋亡刺激蛋白抑制因子(iASPP)、逮捕缺陷(ARD1)蛋白在鼻咽鳞状细胞癌组织中的表达及其临床意义。方法选取济源市第二人民医院2015年1月至2019年1月收集的鼻咽鳞状细胞癌组织标本80例(鼻咽鳞状细胞癌组)、并选取年龄与性别相匹配的40例正常鼻咽部组织(对照组),采用免疫组化染色检测ARD1与iASPP蛋白表达,并分析二者与鼻咽鳞状细胞癌病人的病理学分型、T分期、N分期、临床分期的关系。结果ARD1蛋白、iASPP蛋白在鼻咽鳞状细胞癌组织表达阳性率分别为56.25%、78.75%,在正常鼻咽部组织分别为10.00%、17.50%,鼻咽鳞状细胞癌组织中ARD1蛋白、iASPP蛋白表达阳性率均高于对照组(P<0.05);T3和T4分期鼻咽鳞状细胞癌组织中的ARD1蛋白、iASPP蛋白表达阳性率高于T1和T2分期(P<0.05),N2和N3分期鼻咽鳞状细胞癌组织中的ARD1蛋白、iASPP蛋白表达阳性率高于N0和N1分期(P<0.05),Ⅲ期和Ⅳ期的鼻咽鳞状细胞癌组织中的ARD1蛋白、iASPP蛋白阳性率均分别的高于Ⅰ期和Ⅱ期(P<0.05)。不同病理学分型的鼻咽鳞状细胞癌组织中的ARD1蛋白、iASPP蛋白阳性率均差异无统计学意义(P>0.05)。结论鼻咽鳞状细胞癌组织中的ARD1蛋白、iASPP蛋白表达上调显著,并且与鼻咽鳞状细胞癌的T分期、N分期及临床分期可能具有相关性,其可能在鼻咽癌的发生及进展中共同发挥调控作用。Objective To explore the expression of inhibitor of apoptosis stimulating protein(iASPP)and arrest defect(ARD1)protein in nasopharyngeal squamous cell carcinoma tissue and its clinical significance.Methods We selected 80 cases of nasopharyngeal squamous cell carcinoma.tissue collected from January 2015 to January 2019 in Second People’s Hospital of Jiyuan(nasopharyngeal carcinoma group),and selected 40 normal nasopharyngeal tissues(control group)matched with age and gender.Immunohistochemical staining was used to detect the expression of ARD1 and iASPP protein,and the relationship between them was analyzed with pathological type,T stage,N stage and clinical stage of patients with nasopharyngeal squamous cell carcinoma.Results The positive rates of ARD1 protein and iASPP protein expression in nasopharynx squamous cell carcinoma were 56.25%and 78.75%respectively,and in normal nasopharynx were 10.00%and 17.50%respectively.The positive rates of ARD1 protein and iASPP protein in nasopharynx squamous cell carcinoma were higher than those in the control group(P<0.05);the positive rates of ARD1 protein and iASPP protein in T3 and T4 stage nasopharynx squamous cell carcinoma were higher than those in T1 and T2 stage The positive rates of ARD1 protein and iASPP protein in stage N2 and N3 were higher than that in stage N0 and N1(P<0.05).The positive rates of ARD1 protein and iASPP protein in stageⅢandⅣwere higher than that in stageⅠandⅡ(P<0.05).There was no significant difference in the positive rates of ARD1 protein and iASPP protein in nasopharyngeal squamous cell carcinoma of different pathological types(P>0.05).Conclusion The expression of ARD1 protein and iASPP protein in nasopharyngeal squamous cell carcinoma is up-regulated,and may have a certain correlation with T stage,Its may play a regulatory role in the genesis and progression in nasopharyngeal squamous cell carcinoma.

关 键 词：鼻咽肿瘤 癌 鳞状细胞 凋亡刺激蛋白抑制因子 逮捕缺陷1 

分 类 号：R739.63[医药卫生—肿瘤]