生长抑制特异性转录本5和微小RNA-142-3p在重症急性胰腺炎并急性呼吸窘迫综合征病人血清中的表达及临床意义  

Expression and clinical significance of GAS5 and miR-142-3p in serum of patients with SAP and ARDS

作　　者：何亚丽[1] 刘宏[1] HE Yali;LIU Hong(Department of Critical Care Medicine,Pingdingshan First People's Hospital,Pingdingshan,Henan 467000,China)

机构地区：[1]平顶山市第一人民医院重症医学科,河南平顶山467000

出　　处：《安徽医药》2021年第5期975-979,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨生长抑制特异性转录本5(GAS5)和微小RNA-142-3p(miR-142-3p)在重症急性胰腺炎(SAP)合并急性呼吸窘迫综合征(ARDS)病人血清中的表达及临床意义。方法选取2017年2月至2018年12月平顶山市第一人民医院SAP病人83例,采用随机数字表法抽样41例并发ARDS者为ARDS组,42例非ARDS组。另选取同时期40例健康体检者作为健康对照组。实时荧光定量PCR(RT-qPCR)检测病人血清GAS5和miR-142-3p表达水平,Pearson相关性分析SAP合并ARDS病人血清GAS5与miR-142-3p表达相关性,受试者工作特征(ROC)曲线分析血清GAS5和miR-142-3p对SAP合并ARDS的诊断价值,logistic回归分析SAP合并ARDS及其预后的影响因素。结果与健康对照组比较,非ARDS组和ARDS组病人血清GAS5表达升高(P<0.05),miR-142-3p表达水平降低(P<0.05)。与非ARDS组比较,ARDS组病人血清GAS5表达升高(P<0.05),miR-142-3p表达水平降低(P<0.05)。Pearson相关性分析结果显示,SAP合并ARDS病人血清GAS5与miR-142-3p呈负相关(P<0.05)。GAS5与miR-142-3p联合检测诊断SAP合并ARDS的灵敏度、特异度及曲线下面积(AUC)均高于GAS5、miR-142-3p单独诊断(P<0.05)。与SAP合并ARDS存活病人比较,死亡病人血清GAS5表达水平升高(P<0.05),miR-142-3p表达水平降低(P<0.05)。GAS5和miR-142-3p是SAP病人合并ARDS的显著影响因素(P<0.05),也是SAP合并ARDS病人预后的显著影响因素(P<0.05)。结论SAP合并ARDS病人血清中GAS5表达升高,miR-142-3p表达降低,可能为SAP合并ARDS的诊断和预后判断提供了新的生物学指标。Objective to investigate the expression and clinical significance of growth suppression-specific transcription 5(GAS5)and micrna-142-3p(mir-142-3p)in the serum of patients with severe acute pancreatitis(SAP)combined with acute respiratory distress syndrome(ARDS).Methods A total of 41 patients with ARDS were randomly sampled from SAP patients in Pingdingshan First People’s Hospital from February 2017 to December 2018,including the ARDS group and the non-ards group.In addition,40 healthy physical examinees were selected as the healthy control group.Real-time fluorescence quantitative PCR(rt-qpcr)was used to detect the expression levels of serum GAS5 and mir-142-3p in patients with SAP combined with ARDS.Pearson correlation was used to analyze the correlation between the expression levels of serum GAS5 and mir-142-3p in patients with SAP combined with ARDS.ROC curve was used to analyze the diagnostic value of serum GAS5 and mir-142-3p in patients with SAP combined with ARDS.Results Compared with the healthy control group,the expression of serum GAS5 in the non-ARDS group and ARDS group increased(P<0.05),and the expression level of miR-142-3p decreased(P<0.05),and the expression level of mir-142-3p was decreased(P<0.05).Compared with the non-ards group,the expression of serum GAS5 in ARDS group was increased(P<0.05),and the expression level of mir-142-3p was decreased(P<0.05).Pearson correlation analysis results showed that the serum GAS5 of SAP patients with ARDS was negatively correlated with mir-142-3p(P<0.05).The sensitivity,specificity and AUC of both GAS5 and mir-142-3p were higher than that of GAS5 and mir-142-3p alone(P<0.05).Compared with the surviving patients with SAP combined with ARDS,the expression level of serum GAS5in the deceased patients was increased(P<0.05),and the expression level of mir-142-3p was decreased(P<0.05).GAS5 and mir-142-3p were significant factors affecting ARDS in SAP patients combined with ARDS(P<0.05)and the prognosis of SAP patients combined with ARDS(P<0.05).Conclusion The exp

关 键 词：胰腺炎 急性坏死性 呼吸窘迫综合征 成人 生长抑制特异性转录本5(GAS5) 微小RNA-142-3p 诊断 预后 

分 类 号：R576[医药卫生—消化系统] R563.8[医药卫生—内科学]