鱿鱼Ⅱ型胶原免疫调控巨噬细胞的体外研究  

Squid type Ⅱ collagen immunomodulates the activation of macrophages in vitro

作　　者：戴美璐 隋佰延 刘昕[1] 陆华[1] 孙皎[1] Dai Meilu;Sui Baiyan;Liu Xin;Lu Hua;Sun Jiao(Department of Dental Materials,Shanghai Biomaterials Research&Testing Center,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai 200011)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔材料科,上海生物材料研究测试中心,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海200011

出　　处：《口腔材料器械杂志》2021年第2期91-96,共6页Chinese Journal of Dental Materials and Devices

基　　金：国家自然科学基金项目(编号:82072070);国家重大研究开发项目(编号:2018YFC1105202);上海市青年科技英才扬帆计划(编号:19YF1425700)。

摘　　要：目的研究鱿鱼Ⅱ型胶原(SCⅡ)对巨噬细胞表型的调控作用,探讨其对颞下颌关节退行性骨关节炎局部炎症细胞的调控功能。方法提取SCⅡ,建立SCⅡ-巨噬细胞条件培养模型,通过PCR试验和Western Blot试验等,研究巨噬细胞中的M1标志基因(iNOS,IL6,TNF-α,IL1β)、M2标志基因(Arg1,Fizz1,MR,Ym1,IL10)和促成软骨基因(TGF-β1,TGF-β2,TGF-β3,IGF1,IGF2)的表达水平;并初步探索相关机制。结果 SCⅡ能诱导巨噬细胞表达M2型极化标志基因MR、Arg-1、Frizzl以及Yml,且能促进M2巨噬细胞表达促成软骨基因,其机制可能与SCⅡ激活巨噬细胞中的STAT6信号通路有关。结论 SCⅡ能免疫调控巨噬细胞的极化表型、并有可能间接促进软骨细胞分泌软骨基质,这为其用于缓解退行性骨关节炎的应用提供可能。Object The aim of this study was to investigate the modulatory effect of squid type Ⅱ collagen(SCⅡ) on the phenotypes of macrophage, and to discuss its bio-regulatory effect on immune cells involved in degenerative osteoarthritis(OA)-induced cartilage lesions. Methods SCⅡ was extracted from squid cartilage to establish the SCⅡ conditioned macrophage culture model. Its effects on M1/M2 polarization of macrophages, the specific gene expression of M1 macrophage(iNOS, IL-6, TNF-α, IL-1β) and M2 macrophages(Arg-1, Frizzl, MR, Ym1, IL-10)and the pro-chondrogenic genes(TGF-β1, TGF-β2, TGF-β3, IGF1, IGF2) induced by macrophage and the underlying mechanism were investigated in vitro via PCR, and western blot. Results SCⅡ could induce M2 polarization of macrophages and express the marker genes of M2 macrophage(MR,Arg-1,Frizzl and Ym1). Besides, it promoted the expression of pro-chondrogenic genes by macrophage M2 polarization. Conclusions SCⅡ could immunomodulate the activation of macrophages and probably promote excretion of cartilage matrix by indirect manner, showing great potential as a novel biomaterial for relieving OA.

关 键 词：鱿鱼Ⅱ型胶原 退行性骨关节炎 M2型巨噬细胞 免疫调控 

分 类 号：R782.6[医药卫生—口腔医学]