lncRNA PVT1对前列腺癌细胞增殖 及侵袭、迁移的影响  

Effects of lncRNA PVT1 on proliferation,invasion,and migration of prostate cancer cells

作　　者：张建伟[1] 王华东[1] 郭宝印[1] 李海波[1] 孙凤亮[1] ZHANG Jianwei;WANG Huadong;GUO Baoyin;LI Haibo;SUN Fengliang(Tianjin Baodi Hospital,Tianjin 301800,China)

机构地区：[1]天津市宝坻区人民医院,天津301800

出　　处：《山东医药》2021年第10期45-48,共4页Shandong Medical Journal

摘　　要：目的探讨长链非编码RNA(lncRNA)人浆细胞瘤转化迁移基因1(PVT1)对前列腺癌细胞增殖、侵袭和迁移的影响。方法传代培养人前列腺癌细胞DU145、人正常前列腺上皮细胞RWPE-1(以下分别称DU145、RWPE-1细胞),采用RT-qPCR法检测DU145、RWPE-1细胞lncRNA PVT1 mRNA表达。将DU145细胞随机分为观察组和对照组,分别转染lncRNA PVT1 siRNA和Negative Control siRNA,经G418筛选获得稳转细胞株,采用RT-qPCR法检测lncRNA PVT1 mRNA表达,采用Western blotting法检测lncRNA PVT1蛋白表达,采用MTT法检测培养12、24、36、48、60、72 h细胞增殖活性,采用Transwell小室实验检测细胞侵袭能力,采用细胞划痕实验检测细胞迁移能力。结果DU145细胞lncRNA PVT1 mRNA相对表达量明显高于RWPE-1细胞(t=7.938,P<0.01)。观察组lncRNA PVT1 mRNA和蛋白相对表达量均低于对照组(t分别为5.053、18.110,P均<0.01)。观察组培养60、72 h时细胞增殖活性均低于对照组同期(P均<0.01),而两组培养12、24、36、48 h时细胞增殖活性比较差异均无统计学意义(P均>0.05)。观察组穿膜细胞数和细胞迁移率均低于对照组(t分别为6.982、11.782,P均<0.05)。结论前列腺癌细胞lncRNA PVT1高表达,下调lncRNA PVT1表达可抑制前列腺癌细胞增殖、侵袭和迁移。Objective To investigate the effects of long non-coding RNA(lncRNA)human plasmacytoma variant translocation 1(PVT1)on the proliferation,invasion and migration of prostate cancer cells.Methods Human prostate cancer cells DU145 and human normal prostate epithelial cells RWPE-1(hereinafter referred to as DU145 and RWPE-1 cells,respectively)were subcultured.RT-qPCR was used to detect the expression of lncRNA PVT1 mRNA in DU145 and RWPE-1 cells.The DU145 cells were randomly divided into the observation group and control group.They were transfected with lncRNA PVT1 siRNA and Negative Control siRNA,respectively.The stable transfected cell line was screened by G418.The expression of lncRNA PVT1 mRNA was detected by RT-qPCR,and the expression of lncRNA PVT1 protein was detected by Western blotting.The MTT method was used to detect the cell proliferation activity of cells cultured for 12,24,36,48,60,72 h,the Transwell chamber test was used to detect the cell invasion ability,and the cell Scratch test was used to detect the cell migration ability.Results The relative expression of lncRNA PVT1 mRNA in DU145 cells was significantly higher than that in RWPE-1 cells(t=7.938,P<0.01).The relative expression levels of lncRNA PVT1 mRNA and protein in the observation group were lower than those in the control group(t=5.053 and 18.110,respec⁃tively,both P<0.01).The cell proliferation activity of the observation group was lower than that of the control group at 60 and 72 hours of culture(both P<0.01),but there was no statistically significant difference in cell proliferation activity between the two groups when the cells were cultured for 12,24,36,and 48 h(all P>0.05).The number of transmem⁃brane cells and cell migration rate in the observation group were lower than those in the control group(t=6.982 and 11.782,respectively;both P<0.05).Conclusion The expression of lncRNA PVT1 in the prostate cancer cells is high⁃ly expressed,and down-regulating the expression of lncRNA PVT1 can inhibit the proliferation,invasion and migration o

关 键 词：前列腺癌 长链非编码RNA 人浆细胞瘤转化迁移基因1 细胞增殖 细胞侵袭 细胞迁移 

分 类 号：R737.25[医药卫生—肿瘤]