剪接因子MBNL3对胰腺癌细胞恶性生物学行为的影响  

Influence of splicing factor MBNL3 on malignant biology behavior of pancreatic cancer cells

作　　者：郝文真 陈杰民[1] 冯云路[1] 吴晰[1] 王强[1] 吴东[1] 杨爱明[1] HAO Wen-zhen;CHEN Jie-min;FENG Yun-lu;WU Xi;WANG Qiang;WU Dong;YANG Ai-ming(Department of Gastroenterology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital,CAMS & PUMC, Beijing 100730,China)

机构地区：[1]中国医学科学院,北京协和医学院,北京协和医院,疑难重症及罕见病国家重点实验室消化内科,北京100730

出　　处：《基础医学与临床》2021年第5期688-693,共6页Basic and Clinical Medicine

基　　金：北京科技计划项目(Z181100001618013)。

摘　　要：目的探讨MBNL3在胰腺癌细胞中的表达及对其恶性生物学行为的影响。方法收集自2017年至2020年北京协和医院50例胰腺癌石蜡组织样本,采用免疫组织化学法检测MBNL3蛋白表达。胰腺癌细胞经过MBNL3表达或敲低后,采用实时无标记细胞检测技术、平板集落形成技术和Transwell小室法验证MBNL3对细胞增殖、细胞集落形成、迁移和侵袭能力的影响。采用Western blot检测胰腺癌细胞系中MBNL3表达。结果MBNL3在胰腺癌组织着色的总体评分为5.0±2.7,显著高于其所对应癌旁组织的总体评分(2.1±0.6)(P<0.05)。细胞增殖实验显示,MBNL3敲低组增殖曲线低于对照组,MBNL3过表达组增殖曲线高于对照组。平板集落形成实验显示,MBNL3敲低组集落形成个数显著少于对照组(P<0.05),MBNL3过表达组集落形成个数显著多于对照组(P<0.05)。Transwell小室法显示,MBNL3敲低组细胞迁移及侵袭能力显著低于对照组(P<0.05),MBNL3过表达组迁移及侵袭能力显著高于对照组(P<0.05)。结论MBNL3在胰腺癌中高表达,有促进其增殖、迁移和侵袭等恶性生物学行为的作用。Objective To investigate MBNL3 expression and its effect on malignant biology behavior of pancreatic cancer cells.Methods Fifty paraffin samples of pancreas cancer excised during surgery in Peking Union Medical College Hospital from 2017 to 2020 were collected.Immunohistochemistry was conducted to explore expression of MBNL3.Pancreatic cancer cells were treated with MBNL3 knocked down or over-expressed by transfection.The proliferation and invasion or migration in different pancreas cells were examined respectively by real time cellular analysis and Transwell assay.The colony formation in different pancreas cells were examined by plate colony forming test.The MBNL3 expression in cell lines was analyzed by Western blot.Results Overall staining score of MBNL3 in pancreatic cancer tissues was 5.0±2.7,significantly higher than that of adjacent tissues(2.1±0.6)(P<0.05).Cell proliferation experiment showed that 72 h after transfection,MBNL3 knockdown groups grew slower than control group in pancreatic cancer cells,whereas MBNL3 over-expressed groups grew faster than control group.Transwell assay showed that 10 h after transfection,invasion and migration of MBNL3 knockdown groups were weaker than control groups in pancreatic cancer cells(P<0.05),meanwhile MBNL3 over-expressed groups were stronger than control groups in pancreaticcancer cells(P<0.05).Colony forming experiments showed that colony formation number of MBNL3 knockdown groups were less than control groups in pancreatic cancer cells(P<0.05),and the same of MBNL3 over-expressed groups were more than control group in pancreatic cancer cells(P<0.05).ConclusionsMBNL3 is highly expressed in pancreatic cancer tissue,which might take part in regulating the proliferation,invasion and migration of pancreatic cancer cells.

关 键 词：胰腺癌 剪接因子 侵袭 迁移 

分 类 号：R576[医药卫生—消化系统]